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Detection of slow rotational motions of proteins by steady-state phosphorescence anisotropy

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Abstract

WE have used the anisotropy of the phosphorescence from intrinsic chromophores of proteins to detect slow rotational motions of the macromolecules in highly viscous solutions. This method has potential for monitoring the mobility of proteins in structures such as membranes and viruses.

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References

  1. Perrin, F., Phys. Radium, 7, 390–401 (1962); Ann. Phys., X 12, 169–275 (1929).

    Article  Google Scholar 

  2. Weber, G., Biochem. J., 51, 155–167 (1952); Adv. Protein Chem., 8, 415–455 (1953).

    Article  CAS  Google Scholar 

  3. Weber, G., A. Rev. Biophys. Bioengng, 1, 553–570 (1972).

    Article  CAS  Google Scholar 

  4. Wahl, P., C. r. hebd. Séanc. Acad. Sci., Paris, 263 D, 1525–28 (1966).

    CAS  Google Scholar 

  5. Haugland, R. P., and Stryer, L., in Conformation of Biopolymers, 1, (edit. by Ramachandran, G. N.), 321–335 (Academic, New York, 1967).

    Google Scholar 

  6. Tao, T., Biopolymers, 8, 609–632 (1969).

    Article  CAS  Google Scholar 

  7. Rawitch, A. B., Hudson, E., and Weber, G., J. biol. Chem., 244, 6543–6547 (1969).

    CAS  PubMed  Google Scholar 

  8. Saviotti, M. L., and Galley, W. C., Ninth Spectroscopy Symp. of Canada, Montreal (1972); Proc. natn. Acad. Sci. U.S.A., 71, 4154–4158 (1974).

    Google Scholar 

  9. Konev, S. V., in Fluoresence and Phosphorescence of Proteins and Nucleic cids (Plenum, New York, 1967).

    Book  Google Scholar 

  10. Konev, V. S., and Bobrovich, V. P., Biofizika, 10, 813–16 (1965).

    CAS  PubMed  Google Scholar 

  11. Purkey, R. M., and Galley, W. C., Biochemistry, 9, 3569–3574 (1970).

    Article  CAS  Google Scholar 

  12. Azumi, T., and McGlyn, S. P., J. chem. Phys., 37, 2413–2420 (1962).

    Article  ADS  CAS  Google Scholar 

  13. Galley, W. C., in Concepts in Biochemical Fluoresence (edit. by Chen, R. C., and Edelhoch, H.) (Dekker, New York, 1976).

    Google Scholar 

  14. Einstein, A., Ann. Physik., 19, 371–381 (1906).

    Article  ADS  CAS  Google Scholar 

  15. Juliano, R. L., Biochim. biophys. Acta, 300, 341–378 (1973).

    Article  CAS  Google Scholar 

  16. Kefalides, N. A., Biochem. biophys. Res. Commun., 22, 26–32 (1966).

    Article  CAS  Google Scholar 

  17. Douzou, P., Sireix, R., and Travers, F., Proc. natn. Acad. Sci. U.S.A., 45, 66, 787–792 (1970).

    Article  ADS  Google Scholar 

  18. Douzou, P., Biochimie, 53, 17–23 (1971).

    Article  CAS  Google Scholar 

  19. Douzou, P., Hui Bon Hoa, G., and Petsko, G., J. molec. Biol, 96, 367–380 (1974).

    Article  Google Scholar 

  20. Petsko, G. A., J. molec. Biol, 96, 381–392 (1974).

    Article  Google Scholar 

  21. Fung, K. K., and Kalant, N., Biochem. J., 129, 733–741 (1972).

    Article  CAS  Google Scholar 

  22. Neuman, R. E., Archs Biochem., 24, 289–298 (1949).

    CAS  Google Scholar 

  23. Gang, M., and Kalant, M., Lab. Invest., 22, 531–540 (1970).

    CAS  PubMed  Google Scholar 

  24. Spencer, J. H., Cerny, R., Cerny, E., and Delaney, A. D., J. Virol., 10, 134–141 (1972).

    CAS  PubMed  PubMed Central  Google Scholar 

  25. Dodge, J. T., Mitchell, C., and Hanahan, D. J., Archs Biochem. Biophys., 100, 119–130 (1963).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, McGill University, Montreal, Quebec, Canada, H3C 3G1

    GIOVANNI B. STRAMBINI & WILLIAM C. GALLEY

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  1. GIOVANNI B. STRAMBINI
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  2. WILLIAM C. GALLEY
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STRAMBINI, G., GALLEY, W. Detection of slow rotational motions of proteins by steady-state phosphorescence anisotropy. Nature 260, 554–556 (1976). https://doi.org/10.1038/260554a0

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  • DOI: https://doi.org/10.1038/260554a0

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