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Expression of Klebsiella nitrogen fixation genes (nif) in Azotobacter

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Abstract

NITROGEN fixation mutants have been isolated and biochemically characterised in Azotobacter vinelandii1, but the absence of chromosomal gene transfer systems such as conjugation in this genus has prevented a genetic analysis of such mutants. We now report the intergeneric transfer of Klebsiella nif genes carried on the P-type plasmid, RP41 (refs 2 and 3), to Azotobacter, and their expression in this host.

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Authors and Affiliations

  1. ARC Unit of Nitrogen Fixation, University of Sussex, Brighton, BN1 9QJ, UK

    F. C. CANNON & J. R. POSTGATE

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  1. F. C. CANNON
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  2. J. R. POSTGATE
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CANNON, F., POSTGATE, J. Expression of Klebsiella nitrogen fixation genes (nif) in Azotobacter. Nature 260, 271–272 (1976). https://doi.org/10.1038/260271a0

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  • DOI: https://doi.org/10.1038/260271a0

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