Skip to main content
SpringerLink
Log in

Half life of yeast messenger RNA

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

WE have determined the half life of Saccharomyces cerevisiae messenger RNA (mRNA) and its associated poly (A) sequences, from the kinetics of their labelling with 14C-adenine. Corrections have been made for changes in the ATP pool specific activity during the labelling period. This method of determining the mRNA half life is preferred to observing the decay of mRNA in the presence of inhibitors because changes in the cell metabolism caused by an inhibitor may also change the mRNA half life1. One of our purposes in determining these half lives is for their use in the characterisation of temperature-sensitive mutants which are defective in macromolecular synthesis2. For this reason we have determined the half lives of mRNA and poly(A) in two different conditions, at 23 and 36 °C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Singer, R. H., and Penman, S., Nature, 240, 100 (1972).

    Article  ADS  CAS  Google Scholar 

  2. Hartwell, L. H., J. Bact., 93, 1162 (1967).

    Google Scholar 

  3. Kudrna, R., and Edlin, G., J. Bact., 121, 740 (1975).

    CAS  PubMed  Google Scholar 

  4. McLaughlin, C. S., Warner, J. R., Edmonds, M., Wakazato, H., and Vaughan, M. H., J. biol Chem., 248, 1466 (1973).

    CAS  PubMed  Google Scholar 

  5. Hartwell, L. H., Hutchinson, H. T., Holland, T. M., and McLaughlin, C. S., Molec. gen. Genet., 106, 347 (1970).

    Article  CAS  Google Scholar 

  6. Tønnesen, T., and Friesen, J. D., J. Bact., 115, 889 (1973).

    PubMed  Google Scholar 

  7. Mangearotti, G., and Schlessinger, D., J. molec. Biol., 29, 395 (1967).

    Article  Google Scholar 

  8. Greenberg, J. R., Nature, 240, 103 (1973).

    Google Scholar 

  9. Nierlich, D. P., and Vietmetter, W., J. molec. Biol., 32, 135 (1968).

    Article  CAS  Google Scholar 

  10. Neuhard, J., Randrath, E., and Randerath, K., Analyt. Biochem., 13, 211 (1965).

    Article  CAS  Google Scholar 

  11. Mowbray, S., thesis, Univ. California, Los Angeles (1971).

  12. Hartwell, L. H., and McLaughlin, C. S., Proc. natn. Acad. Sci., U.S.A., 62, 468 (1969).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Author notes

  1. N. S. PETERSEN

    Present address: Biology Department, City of Hope National Medical Center, Duarte, California, 91010

Authors and Affiliations

  1. Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California, 92264

    N. S. PETERSEN, C. S. MCLAUGHLIN & D. P. NIERLICH

  2. Department of Bacteriology and Molecular Biology Institute, University of California, Los Angeles Los Angeles, California, 90024

    C. S. MCLAUGHLIN & D. P. NIERLICH

Authors
  1. N. S. PETERSEN
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. S. MCLAUGHLIN
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. P. NIERLICH
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

PETERSEN, N., MCLAUGHLIN, C. & NIERLICH, D. Half life of yeast messenger RNA. Nature 260, 70–72 (1976). https://doi.org/10.1038/260070a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/260070a0

  • Springer Nature Limited

This article is cited by

Search

Navigation