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Haploid mouse blastocysts developed from bisected zygotes

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Abstract

INTEREST in haploid mouse embryos as a material for studying gene action in early development and as a potential source of haploid cell lines has stimulated a number of attempts to produce this condition experimentally. We describe here a new method of producing haploid mouse embryos by bisecting fertilised eggs. Morphologically normal haploid and haplo-diploid mosaic blastocysts were formed after insertion of such fragments to empty zonae and transfer to the oviduct.

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Author notes

  1. A. K. TARKOWSKI: On leave from the Department of Embryology, Zoological Institute, University of Warsaw.

Authors and Affiliations

  1. Department of Zoology, University of Oxford, Oxford, UK

    A. K. TARKOWSKI & J. ROSSANT

Authors
  1. A. K. TARKOWSKI
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  2. J. ROSSANT
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TARKOWSKI, A., ROSSANT, J. Haploid mouse blastocysts developed from bisected zygotes. Nature 259, 663–665 (1976). https://doi.org/10.1038/259663a0

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  • DOI: https://doi.org/10.1038/259663a0

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