Abstract
THE 33258 Hoechst fluorescence1 and 33258 Hoechst–Giemsa2 methods for differential staining of bromodeoxy-uridine (BrdU)-incorporated chromatids are attractive because of their rapidity and high sensitivity in revealing small sister chromatid exchanges which escape detection by autoradiography. Since carcinogen-induced chromatid breaks are associated with sister chromatid exchanges3 and breakage formation parallels carcinogenesis in typical cases4,5, these methods are essential for understanding the role of chromosome aberrations in carcinogenesis. We show here that other photosensitive dyes such as thionin can replace 33258 Hoechst in the latter method and that the mechanisms of the differential Giemsa method involves photolysis of the BrdU-substituted DNA combined with these photosensitive dyes.
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SUGIYAMA, T., GOTO, K. & KANO, Y. Mechanism of differential Giemsa method for sister chromatids. Nature 259, 59–60 (1976). https://doi.org/10.1038/259059a0
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DOI: https://doi.org/10.1038/259059a0
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