Skip to main content
SpringerLink
Log in

Mediation of serum-induced changes in cyclic nucleotide levels in cultured fibroblasts by cyclic nucleotide phosphodiesterase

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

CELL cultures of untransformed fibroblasts can be maintained in a stationary or relatively quiescent state by adjusting serum concentrations to suboptimal levels1. Addition of excess serum to quiescent cell cultures causes a rapid decrease in the intracellular concentration of cyclic AMP and a rapid increase in cyclic GMP levels2–5. These alterations in cyclic nucleotide concentrations precede the changes in macromolecular synthesis (such as RNA, protein and DNA synthesis) and growth induced by serum, and constitute one of the earliest steps in the sequence of events before the initiation of growth. The mechanism responsible for altering cyclic nucleotide concentrations in response to serum or other growth stimuli has not been elucidated however. We show here that a rapid alteration in cyclic nucleotide phosphodiesterase activity occurs when serum is added to quiescent fibroblasts and demonstrate that this alteration could mediate the changes in cyclic nucleotide levels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Temin, H. M., Pierson, R. W., and Dulab, N. C., in Growth, Nutrition and Metabolism of Cells in Culture (edit. by Rothblat, G. H., and Cristofals, V. J.), 50–82 (Academic London, 1972).

    Google Scholar 

  2. Sheppard, J. R., Nature new Biol., 236, 14–16 (1972).

    Article  CAS  Google Scholar 

  3. Seifert, W., and Paul, D., Nature new Biol., 240, 281–283 (1972).

    Article  CAS  Google Scholar 

  4. Seifert, W. E., and Rudland, P. S., Nature, 248, 138–140 (1974).

    Article  ADS  CAS  Google Scholar 

  5. Rudland, P. S., Seeley, M., and Seifert, W., Nature, 251, 417–419 (1974).

    Article  ADS  CAS  Google Scholar 

  6. Lineweaver, H., and Burk, D., J. Am. chem. Soc., 56, 658–666 (1934).

    Article  CAS  Google Scholar 

  7. Schröder, J., and Plagemann, P. G. W., J. natn. Cancer Inst., 46, 423–429 (1971).

    Google Scholar 

  8. Pledger, W. J., Thompson, W. J., and Strada, S. J., J. Cyclic Nucl. Res. (in the press).

  9. Thompson, W. J., and Appleman, M. M., Biochemistry, 10, 311–316 (1971).

    Article  CAS  Google Scholar 

  10. Thompson, W. J., and Appleman, M. M., J. biol. Chem., 246, 3145–3150 (1971).

    CAS  PubMed  Google Scholar 

  11. Steiner, A. L., Parker, C. W., and Kipnis, D. M., J. biol. Chem., 247, 1106–1113 (1972).

    CAS  PubMed  Google Scholar 

  12. Thompson, W. J., and Williams, R. H., Archs biochem. Biophys., 165, 468–477 (1974).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The University of Texas Medical School at Houston, Program in Pharmacology, Houston, Texas, 77025

    W. J. PLEDGER, W. J. THOMPSON & S. J. STRADA

Authors
  1. W. J. PLEDGER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. J. THOMPSON
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. J. STRADA
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

PLEDGER, W., THOMPSON, W. & STRADA, S. Mediation of serum-induced changes in cyclic nucleotide levels in cultured fibroblasts by cyclic nucleotide phosphodiesterase. Nature 256, 729–731 (1975). https://doi.org/10.1038/256729a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/256729a0

  • Springer Nature Limited

This article is cited by

Search

Navigation