Abstract
NUMEROUS attempts have been made to deduce a secondary structure of 5S RNA1–4. Differing approaches have yielded various structures, but each turns out to be inconsistent with either physical, chemical, biochemical, or comparative evidence. Clearly the situation is not analogous to that which obtained for transfer RNA (tRNA). There is, however, at least one fundamental difference between the two molecules. The functional 5S RNA molecule is at all times an integral part of the 50S ribosomal subunit, whereas tRNA is a transient inhabitant of the ribosome milieu. Thus, it is possible that functional conformations of the 5S RNA molecule need only exist in the context of the ribosome, and consequently, may only exist therein. Thus, the interpretation of experimental investigations on the isolated 5S RNA molecule may have inherent difficulties.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Jordan, B. R., Galling, G., and Jourdan, R., J. molec. Biol., 87, 205–225 (1974).
Kearns, D. R., and Wong, Y. P., J. molec. Biol., 87, 755–774 (1974).
Lewis, J. B., and Doty, P., Nature, 255, 510–512 (1970).
Cantor, C. R., Proc. natn. Acad. Sci. U.S.A., 59, 478–483 (1968).
Richards, E. G., Eur. J. Biochem., 10, 36–42 (1969).
Wrede, P., and Erdmann, FEBS Lett., 33, 315–319 (1973).
Bellemare, G., Vigne, R., and Jordan, B. R., Biochimie, 55, 29–35 (1973).
Pribula, C. D., Fox, G. E., Woese, C. R., Sogin, M., and Pace, N. R., FEBS Lett., 44, 322–323 (1974).
Woese, C. R., Pribula, C. D., Fox, G. E., and Zablen, L., J. molec. Evol., 5 (in the press).
Tinoco, I., Jr, Uhlenbeck, O. C., and Levine, M. D., Nature, 230, 362–367 (1971).
Brownlee, G. G., Sanger, F., and Barrell, B. G., J. molec. Biol., 34, 379–412 (1968).
Siddiqui, M. A. Q., J. Sci. Ind. Res., 32, 34–44 (1973).
Tinoco, I., Jr., et al., Nature new Biol., 246, 40–41 (1973).
Erdmann, V. A., Sprinzl, M., and Pongs, O., Biochem. biophys. Res. Commun., 54, 942–948 (1973).
Dube, S. K., FEBS Lett., 36, 39–42 (1973).
Richter, D., Erdmann, V. A., and Sprinzl, M., Nature new Biol., 246, 132–135 (1973).
Nishikawa, K., and Takemura, S., FEBS Lett., 40, 106–109 (1974).
Hindley, J., and Page, S. M., FEBS Lett., 26, 157–160 (1972).
Gray, P. N., Bellemare, G., and Monier, R., FEBS Lett., 24, 156–159 (1972).
Gray, P. N., Bellemare, G., Monier, R., Garrett, R. A., and Stöffler, G., J. molec. Biol., 77, 133–151 (1973).
Sogin, M. L., and Pace, N. R., Nature, 252, 598–600 (1974).
Pace, N. R., Pato, M. L., McKibbin, J., and Radcliffe, C. W., J. molec. Biol., 75, 619–631 (1973).
Lewis, J. B., and Doty, P., Nature, 225, 510–512 (1970).
Aubert, M., Scott, J. F., Reynier, M., and Monier, R., Biochemistry, 61, 292–299 (1968).
Vigne, R., and Jordan, B. R., Biochimie, 53, 981–986 (1971).
Haselkorn, R., and Rothman-Denes, L. B., A. Rev. Biochem., 42, 397–438 (1973).
Mirzabekov, A. D., and Griffen, B. E., J. molec. Biol., 72, 633–643 (1973).
Aubert, M., Bellemare, G., and Monier, R., Biochimie, 55, 135–142 (1973).
Jordan, B. R., J. molec. Biol., 55, 423–439 (1971).
Connors, P. G., and Beeman, W. W., J. molec. Biol., 71, 31–37 (1972).
Forget, B. G., and Weissman, S. M., J. biol. Chem., 244, 3148–3165 (1969).
Dubuy, B., and Weissman, S. M., J. biol. Chem., 246, 747–761 (1971).
Brownlee, G. G., Cartwright, E., McShane, T., and Williamson, R., FEBS Lett., 25, 8–20 (1972).
Marotta, C. A., Levy, C. C., Weissman, S. M., and Varricchio, F., Biochemistry. 12, 2901–2904 (1973).
Cory, M. J., Payne, P. I., and Dyer, T. A., FEBS Lett., 46, 63–66 (1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fox, G., WOESE, C. 5S RNA secondary structure. Nature 256, 505–507 (1975). https://doi.org/10.1038/256505a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/256505a0
- Springer Nature Limited
This article is cited by
-
Perspectives on Cultivation Strategies of Archaea
Microbial Ecology (2020)
-
Identification of 15 candidate structured noncoding RNA motifs in fungi by comparative genomics
BMC Genomics (2017)
-
The Origin of the 5S Ribosomal RNA Molecule Could Have Been Caused by a Single Inverse Duplication: Strong Evidence from Its Sequences
Journal of Molecular Evolution (2012)
-
A PCR based SNPs marker for specific characterization of English walnut (Juglans regia L.) cultivars
Molecular Biology Reports (2011)
-
A Model of the Origin of the 5S Ribosomal RNA Molecule
Journal of Molecular Evolution (2010)