Skip to main content
SpringerLink
Log in

Continuous cultures of fused cells secreting antibody of predefined specificity

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

THE manufacture of predefined specific antibodies by means of permanent tissue culture cell lines is of general interest. There are at present a considerable number of permanent cultures of myeloma cells1,2 and screening procedures have been used to reveal antibody activity in some of them. This, however, is not a satisfactory source of monoclonal antibodies of predefined specificity. We describe here the derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies. The cell lines are made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor. To understand the expression and interactions of the Ig chains from the parental lines, fusion experiments between two known mouse myeloma lines were carried out.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Potter, M., Physiol. Rev., 52, 631–719 (1972).

    Article  CAS  Google Scholar 

  2. Horibata, K., and Harris, A. W., Expl Cell Res., 60, 61–70 (1970).

    Article  CAS  Google Scholar 

  3. Milstein, C., and Munro, A. J., in Defence and Recognition (edit. by Porter, R. R.), 199–228 (MTP Int. Rev. Sci., Butterworth, London, 1973).

    Google Scholar 

  4. Cotton, R. G. H., and Milstein, C., Nature, 244, 42–43 (1973).

    Article  ADS  CAS  Google Scholar 

  5. Schwaber, J., and Cohen, E. P., Proc. natn. Acad. Sci. U.S.A., 71, 2203–2207 (1974).

    Article  ADS  CAS  Google Scholar 

  6. Littlefield, J. W., Science, 145, 709 (1964).

    Article  ADS  CAS  Google Scholar 

  7. Svasti, J., and Milstein, C., Biochem. J., 128, 427–444 (1972).

    Article  CAS  Google Scholar 

  8. Milstein, C., Adetugbo, K., Cowan, N. J., and Secher, D. S., Progress in Immunology, II, 1 (edit. by Brent, L., and Holborow, J.), 157–168 (North-Holland, Amsterdam, 1974).

    Google Scholar 

  9. Harris, H., and Watkins, J. F., Nature, 205, 640–646 (1965).

    Article  ADS  CAS  Google Scholar 

  10. Awdeh, A. L., Williamson, A. R., and Askonas, B. A., Nature, 219, 66–67 (1968).

    Article  ADS  CAS  Google Scholar 

  11. Milstein, C., Brownlee, G. G., Cartwright, E. M., Jarvis, J. M., and Proudfoot, N. J., Nature, 252, 354–359 (1974).

    Article  ADS  CAS  Google Scholar 

  12. Frangione, B., and Milstein, C., Nature, 244, 597–599 (1969).

    Article  ADS  Google Scholar 

  13. Jerne, N. K., and Nordin, A. A., Science, 140, 405 (1963).

    Article  ADS  Google Scholar 

  14. Cotton, R. G. H., Secher, D. S., and Milstein, C., Eur. J. Immun., 3, 135–140 (1973).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    G. KÖHLER & C. MILSTEIN

Authors
  1. G. KÖHLER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. MILSTEIN
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

KÖHLER, G., MILSTEIN, C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495–497 (1975). https://doi.org/10.1038/256495a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/256495a0

  • Springer Nature Limited

This article is cited by

Search

Navigation