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Existence and possible roles of transcriptional barriers in T7 DNA early region as shown by electron microscopy

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Abstract

Transcription of T7 DNA in vitro by Escherichia coli RNA polymerase in the absence or presence of termination factor ρ has been studied extensively using electron microscopy. It is demonstrated that sequences located between the early genes behave as transient transcriptional barriers in the absence of ρ and are recognised both as ρ-dependent terminators and as RNaseIII cleavage sites.

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Authors and Affiliations

  1. Service de Biochimie (142), Département de Biologie CEN Saclay, BP 2 91190, Gif/Yvette, France

    Jean-Luc Darlix & Mireille Horaist

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  1. Jean-Luc Darlix
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  2. Mireille Horaist
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Darlix, JL., Horaist, M. Existence and possible roles of transcriptional barriers in T7 DNA early region as shown by electron microscopy. Nature 256, 288–292 (1975). https://doi.org/10.1038/256288a0

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  • DOI: https://doi.org/10.1038/256288a0

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