Abstract
Unmethylated reovirus and VSV mRNAs are specifically methylated to form 5′-terminal structures of the type, m7G (5′)ppp (5′) N by protein synthesizing extracts prepared from wheat germ and mouse L cells. Reticulocyte mRNA also contains 5′-terminal m7G. mRNAs having 5′-terminal m7G stimulate protein synthesis in vitro. Removal of m7G by β-elimination abolishes translation of the mRNAs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Furuichi, Y., Morgan, M., Muthukrishnan, S., and Shatkin, A. J., Proc. natn Acad. Sci. U.S.A., 72, 362–366 (1975).
Abraham, G., Rhodes, D. P., and Banerjee, A. K., Cell (in the press).
Both, G. W., Lavi, S., and Shatkin, A. J., Cell, 4, 173–180 (1975).
Both, G. W., Moyer, S., and Banerjee, A. K., Proc. natn. Acad. Sci. U.S.A., 72, 274–278 (1975).
Both, G. W., Banerjee, A. K., and Shatkin, A. J., Proc. natn. Acad. Sci. U.S.A. (in the press).
Shatkin, A. J., Proc. natn. Acad. Sci. U.S.A., 71, 3204–3207 (1974).
Furuichi, Y., Muthukrishnan, S., and Shatkin, A. J., Proc. natn. Acad. Sci. U.S.A., 72, 742–745 (1975).
Abraham, G., Rhodes, D. P., and Banerjee, A. K., Nature, 255, 37–40 (1975).
McDowell, M. J., Joklik, W. K., Villa-Komaroff, L., and Lodish, H. F., Proc. natn. Acad. Sci. U.S.A., 69, 2649–2653 (1972).
Graziadei, W. D., Roy, D., Konigsberg, W., and Lengyel, P., Archs Biochem. Biophys., 158, 266–275 (1973).
Hunt, J. A., Biochem. J., 120, 353–363 (1970).
Miura, K.-I., Watanabe, K., Sugiura, M., and Shatkin, A. J., Proc. natn. Acad. Sci. U.S.A., 71, 3979–3983 (1974).
Furuichi, Y., and Miura, K.-I., Nature, 253, 374–375 (1975).
Urushibara, T., Furuichi, Y., Nishimura, C., and Miura, K.-I., FEBS Lett., 49, 385–389 (1975).
Wei, C. W., and Moss, B., Proc. natn. Acad. Sci. U.S.A., 72, 318–322 (1975).
Lavi, S., and Shatkin, A. J., Proc. natn. Acad. Sci. U.S.A. (in the press).
Furuichi, Y., et al., Proc. natn. Acad. Sci. U.S.A. (in the press).
Reddy, T., Ro-Choi, T. S., Henning, D., and Busch, H., J. biol. Chem., 249, 6486–6494 (1974).
Rottman, F., Shatkin, A. J., and Perry, R. P., Cell, 3, 197–199 (1974).
Rhodes, D. P., Moyer, S. A., and Banerjee, A. K., Cell, 3, 327–333 (1974).
Aviv, H., and Leder, P., Proc. natn. Acad. Sci. U.S.A., 69, 1408–1412 (1972).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Muthukrishnan, S., Both, G., Furuichi, Y. et al. 5′-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation. Nature 255, 33–37 (1975). https://doi.org/10.1038/255033a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/255033a0
- Springer Nature Limited
This article is cited by
-
A noval prognostic signature of the N7-methylguanosine (m7G)-related miRNA in lung adenocarcinoma
BMC Pulmonary Medicine (2023)
-
Hydrogel armed with Bmp2 mRNA-enriched exosomes enhances bone regeneration
Journal of Nanobiotechnology (2023)
-
Biological roles of RNA m7G modification and its implications in cancer
Biology Direct (2023)
-
RNA modification in cardiovascular disease: implications for therapeutic interventions
Signal Transduction and Targeted Therapy (2023)
-
TMEM11 regulates cardiomyocyte proliferation and cardiac repair via METTL1-mediated m7G methylation of ATF5 mRNA
Cell Death & Differentiation (2023)