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Cyclic AMP and the production of sex pili by E. coli K-12 carrying derepressed sex factors

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Abstract

THE filamentous appendages, known as sex pili, determined by F-like and I-like sex factors in enterobacteria, have an essential function in conjugation1. But neither the exact nature of their function, nor their mode of formation is yet known. The number of sex pili ordinarily seen in cultures of Escherichia coli K-12 carrying derepressed F-like or I-like sex factors2 (where pilus production is not limited by specific repression) seldom exceeds an average of 1–2 per bacterium. With I-like pili, however, the numbers can be increased 50-fold or more by exposing the bacteria to either antiserum reacting with the pili3, or vigorous washing4.

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References

  1. Meynell, G. G., Bacterial Plasmids (Macmillan, London, 1972).

    Google Scholar 

  2. Meynell, E., Meynell, G. G., and Datta, N., Bact. Rev., 32, 55–83 (1968).

    PubMed  CAS  Google Scholar 

  3. Lawn, A. M., and Meynell, E., Nature, 235, 441–442 (1972).

    Article  ADS  CAS  Google Scholar 

  4. Lawn, A. M., and Meynell, E., J. gen. Microbiol., 86, 188–190 (1975).

    Article  CAS  Google Scholar 

  5. Stocker, B. A. D., Smith, S. M., and Ozeki, H., J. gen. Microbiol., 30, 201–221 (1963).

    Article  Google Scholar 

  6. Lawn, A. M., and Meynell, E., J. Hyg., Camb., 68, 683–694 (1970).

    CAS  Google Scholar 

  7. Hardy, K. G., Meynell, G. G., Dowman, J. E., and Spratt, B. G., Molec. gen. Genet., 125, 217–230 (1973).

    Article  CAS  Google Scholar 

  8. Perlman, R. L., and Pastan, I., Biochem. biophys. Res. Commun., 37, 151–157 (1969).

    Article  CAS  Google Scholar 

  9. Emmer, M., de Crombrugghe, B., Pastan, I., and Perlmen, R., Proc. natn. Acad. Sci. U.S.A., 66, 480–487 (1970).

    Article  ADS  CAS  Google Scholar 

  10. Fox, C. F., and Wilson, G., Proc. natn. Acad. Sci. U.S.A., 59, 988–995 (1968).

    Article  ADS  CAS  Google Scholar 

  11. Harwood, C. R., and Meynell, G. G., Biochem. biophys. Res. Commun., 63, 535–540 (1975).

    Article  CAS  Google Scholar 

  12. Yokota, T., and Gots, J. S., J. Bact., 103, 513–516 (1970).

    Article  CAS  Google Scholar 

  13. Silverstone, A. E., Goman, M., and Scaife, J. G., Molec. gen. Genet., 118, 223–234 (1972).

    Article  CAS  Google Scholar 

  14. Frydman, A., and Meynell, E., Genet. Res., Camb., 14, 315–332 (1969).

    Article  CAS  Google Scholar 

  15. Meynell, E., and Cooke, M., Genet. Res., Camb., 14, 309–313 (1969).

    Article  CAS  Google Scholar 

  16. Rickenberg, H. V., A. Rev. Microbiol., 28, 353–369 (1974).

    Article  CAS  Google Scholar 

  17. Broman, R. L., Dobrogosz, W. J., and White, D. C., Archs. Biochem. Biophys., 162, 595–601 (1974).

    Article  CAS  Google Scholar 

  18. Brinton, C. C., Crit. Rev. Microbiol., 1, 105–160 (1971).

    Article  ADS  Google Scholar 

  19. Makman, R. S., and Sutherland, E. W., J. biol. Chem., 240, 1309–1314 (1965).

    PubMed  CAS  Google Scholar 

  20. Brodt, P., Leggett, F., and Iyer, R., Nature, 249, 856–858 (1974).

    Article  ADS  CAS  Google Scholar 

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HARWOOD, C., MEYNELL, E. Cyclic AMP and the production of sex pili by E. coli K-12 carrying derepressed sex factors. Nature 254, 628–630 (1975). https://doi.org/10.1038/254628a0

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