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DNA polymerase activity from two temperature-sensitive mutants of Rous sarcoma virus is thermolabile

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Abstract

Temperature sensitivity of growth and transformation by certain early Rous sarcoma virus mutants correlates with thermolability of their virion-associated DNA polymerase. The enzyme is therefore encoded by viral RNA and is required for provirus synthesis.

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References

  1. Wyke, J. A., Virology, 52, 587 (1973).

    Article  CAS  Google Scholar 

  2. Linial, M., and Mason, W. S., Virology, 53, 258 (1973).

    Article  CAS  Google Scholar 

  3. Vogt, P. K., Weiss, R. A., and Hanafusa, H., J. Virol, 13, 551 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Temin, H. M., and Baltimore, D., in Advances in virus research, 17, 129 (Academic Press, New York, 1972).

    Google Scholar 

  5. Baltimore, D., and Smoler, D. F., Proc. natn. Acad. Sci. U.S.A., 68, 1507 (1971).

    Article  ADS  CAS  Google Scholar 

  6. Verma, I. M., and Baltimore, D., in Methods in enzymology (edit. by Grossman, L., and Moldave, K.), 29, 125 (Academic Press, New York, 1973).

    Google Scholar 

  7. Verma, I. M., Meuth, N. L., Fan, H., and Baltimore, D., J. Virol., 13, 1075 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Grandgenett, D. P., Gerard, G. F., and Green, M., Proc. natn. Acad. Sci. U.S.A., 70, 230 (1973).

    Article  ADS  CAS  Google Scholar 

  9. Kacian, D. L., Watson, K. F., Burny, A., and Spiegelman, S., Biochim. biophys. Acta, 246, 365 (1971).

    Article  CAS  Google Scholar 

  10. Faras, A. J., Taylor, J. M., McDonald, J. P., Levinson, W. E., and Bishop, J. M., Biochemistry, 11, 2334 (1972).

    Article  CAS  Google Scholar 

  11. Loeb, L. A., J. biol. Chem., 244, 1672 (1969).

    CAS  Google Scholar 

  12. Leis, J., Berkower, I., and Hurwitz, J., in DNA synthesis in vitro (edit. by Wells, R. D., and Inman, R. B.), 287 (University Park Press, Baltimore, 1973).

    Book  Google Scholar 

  13. Kawai, S., and Hanafusa, H., Virology, 49, 37 (1972).

    Article  CAS  Google Scholar 

  14. Vogt, P. K., Virology, 46, 947 (1971).

    Article  CAS  Google Scholar 

  15. The molecular biology of tumour viruses (edit. by Tooze, J.) Ch. 11 (Cold Spring Harbor Laboratory, Cold Spring Harbor, 1973).

  16. Ross, J., Scolnick, E. M., Todaro, G., and Aaronson, S. A., Nature new Biol., 231, 163 (1972).

    Article  Google Scholar 

  17. Baltimore, D., McCaffrey, R., and Smoler, D. F., in Virus research (edit. by Fox, C. F., and Robinson, W. S.), 51 (Academic Press, New York, 1973).

    Google Scholar 

  18. Hanafusa, H., Baltimore, D., Smoler, D. F., Watson, K. F., Yaniv, A., and Spiegelman, S., Science, 177, 1188 (1972).

    Article  ADS  CAS  Google Scholar 

  19. Peebles, P. T., Haapala, D. K., and Gazdar, A. F., J. gen. Virol., 9, 488 (1972).

    CAS  Google Scholar 

  20. May, J. T., Somers, K. D., and Kit, S., J. gen. Virol., 16, 223 (1972).

    Article  CAS  Google Scholar 

  21. Somers, K. D., May, J. T., Kit, S., McCormick, K. J., Hatch, G. G., Stenback, W. A., and Trentin, J. J., Intervirology, 1, 11 (1973).

    Article  CAS  Google Scholar 

  22. Bishop, J. M., Quintrell, N., Medeiros, E., and Varmus, H. E., Cancer (in the press).

  23. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem., 193, 265 (1951).

    CAS  Google Scholar 

  24. Baltimore, D., Huang, A. S., and Stampfer, M., Proc. natn. Acad. Sci. U.S.A., 66, 572 (1970).

    Article  ADS  CAS  Google Scholar 

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Author notes

  1. Inder M. Verma

    Present address: The Salk Institute, PO Box 1809, San Diego, California, 92112

  2. William S. Mason

    Present address: Institute for Cancer Research, 7701 Burholme Avenue, Philadelphia, Pennsylvania, 19111

  3. William S. Mason: Department of Microbiology, University of Southern California, School of Medicine, 2025 Zonal Avenue, Los Angeles, California 90033

Authors and Affiliations

  1. Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts, 02139

    Inder M. Verma, William S. Mason, Stanley D. Drost & David Baltimore

Authors
  1. Inder M. Verma
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  2. William S. Mason
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  3. Stanley D. Drost
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  4. David Baltimore
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Verma, I., Mason, W., Drost, S. et al. DNA polymerase activity from two temperature-sensitive mutants of Rous sarcoma virus is thermolabile. Nature 251, 27–31 (1974). https://doi.org/10.1038/251027a0

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  • DOI: https://doi.org/10.1038/251027a0

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