Abstract
HERPES simplex virus DNA and RNA have been demonstrated in one specimen of cervical carcinoma1 but these experiments are very difficult to reproduce, possibly because sophisticated hybridisation techniques must be applied to large amounts of tumour cells. In hamster fibroblasts transformed2 by herpes simplex virus 2 (HSV-2), viral RNA sequences have been detected3, but not viral DNA; 60% of these transformed cells reacted with a herpesvirus antiserum, using immunofluorescence techniques applied to unfixed cells4, but the background of positive cells was 10%, in control cells not treated with HSV-2. To confirm or complement hydridisation and immunofluorescence techniques, alternative methods may be useful.
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SPRECHER-GOLDBERGER, S., THIRY, L. & VANDENBUSSCHE, P. Use of immune lymphocytes to detect expression of herpetic genome. Nature 250, 678–679 (1974). https://doi.org/10.1038/250678a0
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DOI: https://doi.org/10.1038/250678a0
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