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Isolation of surface immunoglobulins from lymphocytes from chicken thymus and bursa

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Abstract

ALTHOUGH the chicken is more closely related to dinosaurs and crocodiles than to the mouse1, the clear-cut demarcation between thymus-derived (T) and bursa-derived (B) lymphocytes in this bird2,3 offers an excellent system for study of the two lymphocyte types. B cells have been demonstrated to possess surface immunoglobulin3–5, while T cells have not, although it has been established that light chains at least are expressed on specifically activated chicken T cells6. So far, however, intact polypeptide chains have not been demonstrated on T and B cells. We now describe the isolation and partial characterisation of surface immunoglobulins of chicken B and T lymphocytes. We have evidence that both lymphocyte types have surface immunoglobulins consisting of light chains, μ chains and a heavy chain of molecular weight 40,000.

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References

  1. Walker, A. D., Nature, 237, 257 (1972).

    Article  ADS  Google Scholar 

  2. Warner, N. L., Szenberg, A., and Burnet, F. M., Austr. J. exp. biol med. Sci., 40, 373 (1962).

    Article  CAS  Google Scholar 

  3. Kincade, P. W., and Cooper, M. D., J. Immun., 106, 371 (1971).

    CAS  PubMed  Google Scholar 

  4. Rabellino, E., and Grey, H. M., J. Immun., 106, 1418 (1971).

    CAS  PubMed  Google Scholar 

  5. Albini, B., and Wick, G., Int. Arch. Allergy, 44, 804 (1973).

    Article  CAS  Google Scholar 

  6. Theis, G. A., and Thorbecke, G. J., J. Immun., 110, 91 (1973).

    CAS  PubMed  Google Scholar 

  7. Meyer, R. K., Rao, M. A., and Aspinall, R. L., Endocrinology, 64, 890 (1959).

    Article  CAS  Google Scholar 

  8. Marchalonis, J. J., Cone, R. E., and Santer, V., Biochem. J., 124, 921 (1971).

    Article  CAS  Google Scholar 

  9. Cone, R. E., and Marchalonis, J. J., Biochem. J. (in the press).

  10. Laemmli, U. K., Nature, 227, 680 (1970).

    Article  ADS  CAS  Google Scholar 

  11. Leslie, G. A., and Clem, L. W., J. exp. Med., 130, 1337 (1969).

    Article  CAS  Google Scholar 

  12. Martin, L. N., and Leslie, G. A., Proc. Soc. exp. biol. Med., 143, 241 (1973).

    Article  CAS  Google Scholar 

  13. Marchalonis, J. J., Atwell, J. L., and Haustein, D., Biochim. biophys. Acta (in the press).

  14. Vitetta, E. S., Baur, S., and Uhr, J. W., J. exp. Med., 134, 242 (1971).

    Article  CAS  Google Scholar 

  15. Marchalonis, J. J., Cone, R. E., and Atwell, J. L., J. exp. Med., 135, 956 (1972).

    Article  CAS  Google Scholar 

  16. Choi, Y. S., and Good, R. A., J. exp. Med., 135, 1133 (1972).

    Article  CAS  Google Scholar 

  17. Chartrand, S. L., Litman, G. W., Lapointe, N., Good, R. A., and Frommel, D., J. Immun. 107, 1 (1971).

    CAS  PubMed  Google Scholar 

  18. Grey, H. M., J. Immun., 98, 811 (1967).

    CAS  PubMed  Google Scholar 

  19. Marchalonis, J. J., and Cone, R. E., Austr. J. exp. Biol. med. Sci., 51, 461 (1973).

    Article  CAS  Google Scholar 

  20. Marchalonis, J. J., and Cone, R. E., Transplant. Rev., 14, 3 (1973).

    CAS  PubMed  Google Scholar 

  21. Moroz, C., and Hahn, Y., Proc. natn. Acad. Sci. U.S.A., 70, 3716 (1973).

    Article  ADS  CAS  Google Scholar 

  22. Bienenstock, J., Gauldie, J., and Perey, D. Y. E., J. Immun., 111, 1112 (1973).

    CAS  PubMed  Google Scholar 

  23. Morrison, M., and Hultquist, D. E., J. biol. Chem., 238, 2847 (1963).

    Google Scholar 

  24. Cone, R. E., Marchalonis, J. J., and Rolley, R. T., J. exp. Med., 134, 1373 (1971).

    Article  CAS  Google Scholar 

  25. Ladoulis, C. T., Gill, III, T. J., Chen, S.-C., and Misra, D. N., Prog. Allergy (in the press).

  26. Boylston, A. W., and Mowbray, J. F., cited in Greaves, M. F., Owen, J. J. T., and Raff, M. C., T and B lymphocytes, 116 (Excerpta Medica Foundation, Amsterdam, 1973).

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Authors and Affiliations

  1. The Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Victoria, 3050, Australia

    A. SZENBERG, R. E. CONE & J. J. MARCHALONIS

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  1. A. SZENBERG
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  2. R. E. CONE
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  3. J. J. MARCHALONIS
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SZENBERG, A., CONE, R. & MARCHALONIS, J. Isolation of surface immunoglobulins from lymphocytes from chicken thymus and bursa. Nature 250, 418–420 (1974). https://doi.org/10.1038/250418a0

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  • DOI: https://doi.org/10.1038/250418a0

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