Abstract
ONLY one phage gene, A, has been shown to be necessary for the replication of ΦX174 double-stranded replicative form (RF) DNA1–3. The host genes required have yet to be fully determined although one host mutation, rep−3, prevents progeny RF synthesis4. Dissection of the characteristics of gene A has shown that it is (a) cis acting5; (b) necessary for the detection of a cis-related viral strand specific discontinuity in the parental RF6,7; (c) the region of the genome which contains the origin of DNA replication8, and (d) a gene from which two proteins, A and A′ (molecular weights 35,000 and 60-65,000, respectively) are synthesised9,10.
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LINNEY, E., HAYASHI, M. Intragenic regulation of the synthesis of ΦX174 gene A proteins. Nature 249, 345–348 (1974). https://doi.org/10.1038/249345a0
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DOI: https://doi.org/10.1038/249345a0
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