Abstract
RHODOPSIN constitutes over 80% of the membrane-bound protein in the outer segment of rod photoreceptors1,2. Absorption of light by rhodopsin leads to large changes in the ionic current of the receptor cell3: excitation of a single rhodopsin molecule transiently (∼1 s) stops about 107 Na ions from flowing across the plasma membrane of the outer segment4,5. The mechanism by which the excited rhodopsin molecule achieves such large reduction in ionic flux is not understood, partly because natural rhodopsin-containing membranes amenable to the experimental methods required to investigate the transport properties of rhodopsin have not been successfully prepared. As an alternative to natural membranes, attempts to form model membranes which incorporate the properties of the photoreceptor have been made. Takagi et al.6 and Fesenko et al.7 have reported the incorporation of ultrasonicated fragments of rod outer segments into model membranes. In this study we report the successful incorporation of native, purified rhodopsin into bilayer membranes. This should make possible the investigation of the mechanism by which rhodopsin molecules act in the rod photoreceptor.
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Robinson, W. E., Gordon-Walker, A., and Bownds, D., Nature new Biol., 235, 112 (1972).
Heitzmann, H., Nature new Biol., 235, 114 (1972).
Hagins, W. A., Penn, R. D., and Yoshikami, S., Biophys. J., 10, 380 (1970).
Korenbrot, J. I., and Cone, R. A., J. gen. Physiol., 60, 20 (1972).
Korenbrot, J. I., Brown, D. T., and Cone, R. A., J. Cell Biol., 56, 389 (1973).
Tagaki, M., Azuma, K., and Kishimoto, U., Ann. Rep. Biol. Work, Fac. Sci. Osaka Univ., 13, 107 (1965).
Fesenko, E. E., Zhavoronok, A. I., and Fesenko, N. K., Dokl. Akad. Nauk S.S.S.R., 207, 472 (1972).
Hong, K., and Hubbell, W. L., Proc. natn. Acad. Sci. U.S.A., 69, 2617 (1972).
Montal, M., and Mueler, P., Proc. natn. Acad. Sci. U.S.A., 69, 3561 (1972).
McConnell, D., J. Cell Biol., 27, 459 (1965).
Wald, G., and Brown, P. K., J. gen. Physiol., 37, 189 (1953).
Gitler, C., and Montal, M., Biochem. biophys. Res. Commun., 47, 1486 (1972).
Folch-Pi, J., and Lees, M., J. biol. Chem., 191, 807 (1951).
Gitler, C., and Montal, M., FEBS Lett., 28, 329 (1972).
Shichi, H., Lewis, M. S., Irreverre, F., and Stone, A. L., J. biol. Chem., 244, 529 (1969).
Heller, J., and Lawrence, M. A., Biochemistry, 9, 864 (1970).
Steinemann, A., and Stryer, L., Biochemistry, 12, 1499 (1973).
Blasie, J. K., Biophys. J., 12, 191 (1972).
Montal, M., Biochim. biophys. Acta, 298, 750 (1973).
Hanai, T., Haydon, D. A., and Taylor, J., J. theor. Biol., 9, 422 (1965).
Blaurock, A. E., and Wilkins, W. H. F., Nature, 223, 906 (1969).
Blaurock, A. E., and Wilkins, W. H. F., Nature, 236, 313 (1972).
Cone, R. A., Nature new Biol., 236, 39 (1972).
Blasie, J. K., Worthington, C. R., and Dewey, M. M., J. molec. Biol., 39, 407 (1969).
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MONTAL, M., KORENBROT, J. Incorporation of Rhodopsin Proteolipid into Bilayer Membranes. Nature 246, 219–221 (1973). https://doi.org/10.1038/246219a0
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DOI: https://doi.org/10.1038/246219a0
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