Abstract
IT would be useful for many studies of cell–cell interaction (for example, contact inhibition of movement, topoinhibition, electrical coupling) to be able to prepare cell cultures of various defined shapes and sizes. This can be done by protecting the whole surface of the culture substrate, except for desired regions, with a non-adhesive substance. The cells would then adhere and proliferate only in these regions. The protective substance must be (i) non-adhesive for cells; (ii) non-toxic for cells; (iii) insoluble in the media and (iv) strongly attached to the underlying substrate so that neither the size nor shape of adhesive region would change during cultivation. Lieberman et al.1 used dried agar film on a collagen layer as the non-adhesive substance for cardiac muscle cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Lieberman, M., Roggeven, A. E., Purdy, I. E., and Jonson, E. A., Science, 175, 909 (1972).
Folch, J., Less, M., and Stanley, G. H., J. Biol. Chem., 226, 497 (1958).
Mueller, P., Rudin, D. O., Tien, H. Ti., and Wescott, W. C., J. Phys. Chem., 67, 2 (1963).
Earle, W. R., Shilling, E. L., Stark, T. A., Straus, N. P., Brown, M. F., and Shelton, E., J. Nat. Cancer Inst., 4, 165 (1943).
Vasiliev, Ju. M., Gelfand, I. M., Domnina, L. V., and Rappoport, R. I., Exp. Cell Res., 54, 83 (1969).
Vasiliev, Ju. M., Gelfand, I. M., Domnina, L. V., Ivanova, O. Yu., Komm, S. G., and Olshevskaja, L. V., J. Embriol. Exp. Morph., 24, 625 (1970).
Taylor, A. C., Exp. Cell Res., Suppl., 8, 154 (1961).
Liberman, E. A., and Nenashev, V. A., Biophysica, 17, 2 (1972) (in Russian).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
IVANOVA, O., MARGOLIS, L. The Use of Phospholipid Film for Shaping Cell Cultures. Nature 242, 200–201 (1973). https://doi.org/10.1038/242200a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/242200a0
- Springer Nature Limited
This article is cited by
-
Automated detection of patterned single-cells within hydrogel using deep learning
Scientific Reports (2022)
-
Microstamp patterns of biomolecules for high-resolution neuronal networks
Medical & Biological Engineering & Computing (1998)
-
Phospholipase activity in human tumors: Localization by cytochemical staining
Journal of Cancer Research and Clinical Oncology (1984)
-
Mechanism of non-adhesiveness of endothelial and epithelial surfaces
Nature (1978)
-
Chemical and Mechanical Requirements for Fibroblast Adhesion
Nature (1973)