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White-light Three Dimensional Microscopy using Multiple-image Storing and Decoding

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Abstract

THERE is a need in microscopy for high quality imaging of transparent specimens which have a large axial depth compared with the depth of focus. Because of the necessarily limited axial depth of focus of conventional microscopes, when a biologist needs detailed information throughout the thickness of a specimen, without compromising lateral resolution, he has to take a series of successive photographs on separate plates (or film frames).

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Authors and Affiliations

  1. Massachusetts General Hospital, Harvard Medical School, and Shriners Burns Institute, Boston

    J. F. BURKE

  2. State University of New York, Stony Brook

    G. INDEBETOUW

  3. Institut d'Optique, Paris

    G. NOMARSKI

  4. State University of New York, Stony Brook

    G. W. STROKE

  5. Harvard Medical School,

    G. W. STROKE

Authors
  1. J. F. BURKE
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  2. G. INDEBETOUW
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  3. G. NOMARSKI
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  4. G. W. STROKE
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BURKE, J., INDEBETOUW, G., NOMARSKI, G. et al. White-light Three Dimensional Microscopy using Multiple-image Storing and Decoding. Nature 231, 303–306 (1971). https://doi.org/10.1038/231303a0

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  • DOI: https://doi.org/10.1038/231303a0

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