Skip to main content
SpringerLink
Log in

Human Blastocysts grown in Culture

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

WE have already described the culture of cleaving human embryos to the sixteen celled stage1, and we now wish to give details of a few embryos that have developed much further, including two that reached fully developed blastocysts. Methods were similar to those described before. Preovulatory oocytes recovered by laparoscopy2 were fertilized in Bavister's medium3, and transferred after 12–15 h into Ham's F 10 supplemented with human or foetal calf serum. Preliminary details of this work have been presented elsewhere4.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Edwards, R. G., Steptoe, P. C., and Purdy, J. M., Nature, 227, 1307 (1970).

    Article  ADS  CAS  Google Scholar 

  2. Steptoe, P. C., and Edwards, R. G., Lancet, i, 683 (1970).

  3. Bavister, B. D., J. Reprod. Fert., 18, 544 (1969).

    Article  CAS  Google Scholar 

  4. Edwards, R. G., Harold C. Mack Symposium on the Biology of Fertilization and Implantation, Detroit, October 1970 (in the press).

    Google Scholar 

  5. Tarkowski, A. K., Cytogenetics, 5, 394 (1966).

    Article  Google Scholar 

  6. Amoroso, E. C., Memoirs of the Society for Endocrinology, 6, 50 (1959).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Oldham General Hospital, Lancashire, and Physiological Laboratory, University of Cambridge,

    P. C. STEPTOE, R. G. EDWARDS & J. M. PURDY

Authors
  1. P. C. STEPTOE
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. G. EDWARDS
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. M. PURDY
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

STEPTOE, P., EDWARDS, R. & PURDY, J. Human Blastocysts grown in Culture. Nature 229, 132–133 (1971). https://doi.org/10.1038/229132a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/229132a0

  • Springer Nature Limited

This article is cited by

Search

Navigation