Abstract
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure.
Similar content being viewed by others
References
Sarabhai, A. S., Stretton, A. O. W., Brenner, S., and Bolle, A., Nature, 201, 13 (1964).
Kellenberger, E., Virology, 34, 549 (1968).
Baylor, M. B., and Roslansky, P. F., Virology, 40, 251 (1970).
Epstein, R. H., Bolle, A., Steinberg, C. H., Kellenberg, E., Boy de la Tour, E., Chevalley, R., Edgar, R. S., Susman, M., Denhardt, G. H., and Lielausis, J., Cold Spring Harbor Symp. Quant. Biol., 28, 375 (1963).
Laemmli, U. K., Molbert, E., Showe, M., and Kellenberger, E., J. Mol. Biol., 49, 99 (1970).
Laemmli, U. K., Beguin, F., and Gujer-Kellenberger, G., J. Mol. Biol., 47, 69 (1970).
Edgar, R. S., and Wood, W. B., Proc. US Nat. Acad. Sci., 55, 498 (1966).
Davis, B. J., Ann. NY Acad. Sci., 121, 404 (1964).
Maizel, J. V., Fundamental Techniques of Virology (edit. by Habel, K., and Salzman, N. P.), chap. 32, 334 (Academic Press, New York, 1969).
Shapiro, A. L., Viñuela, E., and Maizel, J. V., Biophys. Biochem. Res. Commun., 28, 815 (1967).
Minigawa, T., Virology, 13, 515 (1961).
Hosoda, J., and Levinthal, C., Virology, 34, 709 (1968).
King, J., J. Mol. Biol., 32, 231 (1968).
King, J., FEBS Symp. (edit. by Ochoa, S., Nachmansohn, D., Asensio, C., and Heredia, C. F.), 21, 156 (Academic Press, London, 1970).
Favre, R., Boy de la Tour, E., Segre, N., and Kellenberger, E., J. Ultrastruct. Res., 13, 318 (1965).
Kellenberger, E., Eiserling, F. A., and Boy de la Tour, E., J. Ultrastruct. Res., 21, 335 (1968).
Eddleman, H. L., and Champe, S. P., Virology, 30, 471 (1966).
Sternberg, N., and Champe, S. P., J. Mol. Biol., 46, 337 (1970).
Laemmli, U. K., and Eiserling, F. A., Molec. Gen. Genet., 101, 333 (1968).
Fairbanks, G., Levinthal, C., and Reeder, R. H., Biochem. Biophys. Res. Commun., 20, 393 (1965).
Larcom, L., Bendet, I., and Mumma, S., Virology, 41, 1 (1970).
Weber, K., and Osborn, M., J. Biol. Chem., 244, 4406 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
LAEMMLI, U. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature 227, 680–685 (1970). https://doi.org/10.1038/227680a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/227680a0
- Springer Nature Limited
This article is cited by
-
Inoculation of heavy metal resistant bacteria alleviated heavy metal-induced oxidative stress biomarkers in spinach (Spinacia oleracea L.)
BMC Plant Biology (2024)
-
Glycylglycine promotes the solubility and antigenic utility of recombinant HCV structural proteins in a point-of-care immunoassay for detection of active viremia
Microbial Cell Factories (2024)
-
Proteomic analysis of the developing mammalian brain links PCDH19 to the Wnt/β-catenin signalling pathway
Molecular Psychiatry (2024)
-
Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to β-glucans
Nature Communications (2024)
-
KdpD is a tandem serine histidine kinase that controls K+ pump KdpFABC transcriptionally and post-translationally
Nature Communications (2024)