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Segregation of Transferable R Factors into Escherichia coli Minicells

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Abstract

IT is difficult to isolate the DNA of Escherichia coli episomes because its physical and biological properties are similar to those of the bacterial chromosome. We have solved this problem by isolating the R factor in minicells, tiny DNA-less cells resulting from abnormal cell division of an E. coli mutant1. Minicells have been used as recipients for DNA transfer from normal cells2, but in general, except with F factor alone, the transferred DNA remains as a non-replicating single strand2.

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References

  1. Adler, H. I., Fisher, W. D., Cohen, A., and Hardigree, A. A., Proc. US Nat. Acad. Sci., 57, 321 (1967).

    Article  ADS  CAS  Google Scholar 

  2. Cohen, A., Fisher, W. D., Curtiss, R., and Adler, H. I., Proc. US Nat. Acad. Sci., 61, 61 (1968).

    Article  ADS  CAS  Google Scholar 

  3. Kass, L. R., and Yarmolinsky, M., Proc. US Nat. Acad. Sci. (in the press).

  4. Cohen, A., Fisher, W. D., Curtiss, R., and Adler, H. I., Cold Spring Harbor Symp. Quant. Biol., 33, 635 (1968).

    Article  CAS  PubMed  Google Scholar 

  5. Watanabe, T., and Fukasawa, T., J. Bact., 81, 679 (1961).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Lennox, E. S., Virology, 1, 190 (1955).

    Article  CAS  PubMed  Google Scholar 

  7. Boyce, R. P., and Setlow, R. B., Biochim. Biophys. Acta, 61, 618 (1962).

    CAS  Google Scholar 

  8. Nisioka, T., Mitani, M., and Clowes, R., J. Bact., 97, 376 (1969).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Falkow, S., Haapala, D. K., and Silver, R. P., in Ciba Foundation Symposium on Bacterial Episomes and Plasmids (edit. by Wolstenholme, G. E. W., and O'Connor, M.), 136 (Churchill, London, 1969).

    Google Scholar 

  10. Curtiss, R., Charamella, L. J., Stallions, D. R., and Mays, J. A., Bact. Rev., 32, 320 (1968).

    PubMed  PubMed Central  Google Scholar 

  11. Meynell, E., Meynell, G. G., and Datta, N., Bact. Rev., 32, 55 (1968).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Lederberg, J., and Lederberg, E. M., J. Bact., 63, 399 (1952).

    CAS  PubMed  PubMed Central  Google Scholar 

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Author notes

  1. PAULA NORMAN

    Present address: Kalamazoo College, Kalamazoo, Michigan, 49203

Authors and Affiliations

  1. Laboratory of Biochemical Pharmacology, National Institue of Arthritis and Metabolic Diseases, National Institutets of Health, Bethesda, Maryland, 20014

    STUART B. LEVY & PAULA NORMAN

Authors
  1. STUART B. LEVY
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  2. PAULA NORMAN
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LEVY, S., NORMAN, P. Segregation of Transferable R Factors into Escherichia coli Minicells. Nature 227, 606–607 (1970). https://doi.org/10.1038/227606a0

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  • DOI: https://doi.org/10.1038/227606a0

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