Skip to main content
SpringerLink
Log in

Three Dimensional Reconstructions of Spherical Viruses by Fourier Synthesis from Electron Micrographs

  • Article
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Methods are developed for computing three dimensional density maps from transmission electron micrographs using Fourier transforms. The reconstructions objectively combine data from several different views of one or more particles. When applied to negatively stained spherical viruses, the technique gives clear reconstructions of their surface structures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. De Rosier, D. J., and Klug, A., Nature, 217, 130 (1968).

    Article  ADS  CAS  Google Scholar 

  2. Moore, P. B., Huxley, H. E., and De Rosier, D. J., J. Mol. Biol. (in the press).

  3. Finch, J. T., and Gibbs, A. J., J. Gen. Virol., 6, 141 (1970).

    Article  CAS  Google Scholar 

  4. Crowther, R. A., De Rosier, D. J., and Klug, A., Proc. Roy. Soc., A (in the press).

  5. Caspar, D. L. D., and Klug, A., Cold Spring Harbor Symp. Quant. Biol., 27, 1 (1962).

    Article  CAS  Google Scholar 

  6. Arndt, U. W., Crowther, R. A., and Mallett, J. F. W., J. Sci. Instrum., Series 2, 1, 510 (1968).

    Article  ADS  CAS  Google Scholar 

  7. Klug, A., and Finch, J. T., J. Mol. Biol., 11, 403 (1965).

    Article  CAS  Google Scholar 

  8. Klug, A., and Finch, J. T., J. Mol. Biol., 31, 1 (1968).

    Article  CAS  Google Scholar 

  9. Klug, A., and Caspar, D. L. D., Adv. Virus Res., 7, 225 (1960).

    Article  CAS  Google Scholar 

  10. Finch, J. T., Klug, A., and Leberman, R., J. Mol. Biol. (in the press).

  11. Harrison, S. C., thesis, Harvard Univ. (1967).

  12. Cooley, J. W., and Tukey, J. W., Maths. of Computation, 19, 297 (1965).

    Article  Google Scholar 

  13. Whittaker, E. T., Proc. Roy. Soc. Edinburgh, 35, 181 (1915); Shannon, C. E., Proc. Inst. Radio Eng. New York, 37, 10 (1949).

    Article  Google Scholar 

  14. Sayre, D., Acta Cryst., 5, 843 (1952).

    Article  Google Scholar 

  15. Klug, A., Crick, F. H. C., and Wyckoff, H. W., Acta Cryst., 11, 199 (1958).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. D. J. DE ROSIER

    Present address: Department of Chemistry, University of Texas, Austin, Texas, 78712

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge

    R. A. CROWTHER, LINDA A. AMOS, J. T. FINCH, D. J. DE ROSIER & A. KLUG

Authors
  1. R. A. CROWTHER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. LINDA A. AMOS
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. T. FINCH
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. J. DE ROSIER
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. KLUG
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

CROWTHER, R., AMOS, L., FINCH, J. et al. Three Dimensional Reconstructions of Spherical Viruses by Fourier Synthesis from Electron Micrographs. Nature 226, 421–425 (1970). https://doi.org/10.1038/226421a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/226421a0

  • Springer Nature Limited

This article is cited by

Search

Navigation