Skip to main content
SpringerLink
Log in

Position of the Initiator and Peptidyl Sites in the E. coli Ribosome

  • Article
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

If an initiation complex is formed with E. coli ribosomes, viral messenger RNA and formylmethionyl-tRNA and the unprotected messenger RNA is then digested with ribonuclease, the ribosome is still able to synthesize a pentapeptide fragment. The point of entry for messenger RNA on the ribosome therefore seems to be four codons removed from the initiator site. The same distance was found for the peptidyl site.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Malkin, L. I., and Rich, A., J. Mol. Biol., 26, 329 (1967).

    Article  CAS  Google Scholar 

  2. Takanami, M., and Zubay, G., Proc. US Nat. Acad. Sci., 51, 834 (1964).

    Article  ADS  CAS  Google Scholar 

  3. Takanami, M., Yan, Y., and Jukes, T. H., J. Mol. Biol., 12, 761 (1965).

    Article  CAS  Google Scholar 

  4. Adams, J. M., and Capecchi, M. R., Proc. US Nat. Acad. Sci., 55, 147 (1966).

    Article  ADS  CAS  Google Scholar 

  5. Steitz, J. A., Nature, 224, 957 (1969).

    Article  ADS  CAS  Google Scholar 

  6. Hindley, J., and Staples, D. H., Nature, 224, 964 (1969).

    Article  ADS  CAS  Google Scholar 

  7. Gesteland, R. F., and Boedtker, H., J. Mol. Biol., 8, 496 (1964).

    Article  CAS  Google Scholar 

  8. Gussin, G. N., J. Mol. Biol., 21, 435 (1966).

    Article  CAS  Google Scholar 

  9. Zinder, N. D., Engelhardt, D. L., and Webster, R. E., Cold Spring Harbor Symp. Quant. Biol., 31, 251 (1966).

    Article  CAS  Google Scholar 

  10. Bretscher, M., J. Mol. Biol., 34, 131 (1968).

    Article  CAS  Google Scholar 

  11. Capecchi, M. R., Biochem. Biophys. Res. Commun., 28, 773 (1967).

    Article  CAS  Google Scholar 

  12. Lucas-Lenard, J., and Lipmann, F., Proc. US Nat. Acad. Sci., 55, 1562 (1966).

    Article  ADS  CAS  Google Scholar 

  13. Haenni, A. L., and Lucas-Lenard, J., Proc. US Nat. Acad. Sci., 61, 1363 (1968).

    Article  ADS  CAS  Google Scholar 

  14. Erbe, R. W., Nau, M. M., and Leder, P., J. Mol. Biol., 39, 441 (1969).

    Article  CAS  Google Scholar 

  15. Cahn, F., Schachter, E. M., and Rich, A., Biochim. Biophys. Acta (in the press).

  16. Capecchi, M. R., Proc. US Nat. Acad. Sci., 58, 1144 (1967).

    Article  ADS  CAS  Google Scholar 

  17. Lodish, H., Nature, 220, 345 (1968).

    Article  ADS  CAS  Google Scholar 

  18. Thach, R. E., Dewey, K. F., and Mykolajewycz, N., Proc. US Nat. Acad. Sci., 57, 1103 (1967).

    Article  ADS  CAS  Google Scholar 

  19. Friedman, H., Lu, P., and Rich, A., Nature, 223, 909 (1969).

    Article  ADS  CAS  Google Scholar 

  20. Sheehan, J. C., and Yang, D. H., J. Amer. Chem. Soc., 80, 1154 (1958).

    Article  CAS  Google Scholar 

  21. Schofield, P., and Zamecnik, P. C., Biochim. Biophys. Acta, 155, 410 (1968).

    Article  CAS  Google Scholar 

  22. Sanger, F., Bretscher, M. S., and Hocquard, E. J., J. Mol. Biol., 8, 38 (1964).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. ERNST KUECHLER

    Present address: Institut für Biochemie, University of Vienna, Vienna, 9

Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts

    ERNST KUECHLER & ALEXANDER RICH

Authors
  1. ERNST KUECHLER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. ALEXANDER RICH
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

KUECHLER, E., RICH, A. Position of the Initiator and Peptidyl Sites in the E. coli Ribosome. Nature 225, 920–924 (1970). https://doi.org/10.1038/225920a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/225920a0

  • Springer Nature Limited

This article is cited by

Search

Navigation