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Fractionation of Antibodies against Staphylococcal Nuclease on ‘Sepharose’ Immunoadsorbents

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Abstract

IMMUNIZATION of an animal with a protein antigen usually elicits a mixture of serum antibodies against different determinants on the immunizing protein1. The correlation of amino-acid sequence and three-dimensional conformation of the antigen with its immunochemical behaviour has been facilitated by the use of specific immunoadsorbents. In this technique, the antigen is coupled chemically to an insoluble support, such as bromoacetyl cellulose2,3 and an ethylene–maleic anhydride copolymer4 used in studies of antigenic sites in lysozyme5 and myoglobin6. ‘Sephadex’ and, more recently, ‘Sepharose’ have been introduced as effective supports for attachment of antibodies7, hormones8, enzyme substrates9 and polypeptide fragments10.

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Authors and Affiliations

  1. Laboratory of Chemical Biology, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland, 20014

    GILBERT S. OMENN, DAVID A. ONTJES & CHRISTIAN B. ANFINSEN

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  1. GILBERT S. OMENN
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  2. DAVID A. ONTJES
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  3. CHRISTIAN B. ANFINSEN
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OMENN, G., ONTJES, D. & ANFINSEN, C. Fractionation of Antibodies against Staphylococcal Nuclease on ‘Sepharose’ Immunoadsorbents. Nature 225, 189–190 (1970). https://doi.org/10.1038/225189a0

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