Abstract
By testing indiscriminately several thousand colonies of mutagenized E. coli, a mutant has been isolated that on extraction proves to have less than I per cent of the normal level of DNA polymerase. The mutant multiplies normally but has acquired an increased sensitivity to ultraviolet light.
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References
Lehman, I. R., Bessman, M. J., Simms, E. S., and Kornberg, A., J. Biol. Chem., 233, 163 (1958).
Bonhoeffer, F., Z. Vererbungslehre, 98, 141 (1966).
Buttin, G., and Wright, M., Cold Spring Harbor Symp. Quant. Biol., 33, 259 (1968).
Fangman, W. L., and Novick, A., Genetics, 60, 1 (1968).
Gross, J. D., Karamata, D., and Hempstead, P. G., Cold Spring Harbor Symp. Quant. Biol., 33, 307 (1968).
Hirota, Y., Ryter, A., and Jacob, F., Cold Spring Harbor Symp. Quant. Biol., 33, 677 (1968).
Couch, J., and Hanawalt, P. C., Biochem. Biophys. Res. Commun., 29, 779 (1967).
Kanner, L., and Hanawalt, P. C., Biochim. Biophys. Acta, 157, 532 (1968).
Kornberg, A., Science, 163, 1410 (1969).
Gesteland, R. F., J. Mol. Biol., 16, 67 (1966).
Godson, G. N., and Sinsheimer, R. L., Biochim. Biophys. Acta, 149, 476 (1967).
Billen, D., Biochim. Biophys. Acta, 68, 342 (1963).
Cohen, A., Fisher, W. D., Curtiss, R., and Adler, H. I., Cold Spring Harbor Symp. Quant. Biol., 33, 635 (1968).
Hirota, Y., Jacob, F., Ryter, A., Buttin, G., and Nakai, T., J. Mol. Biol., 35, 175 (1968).
Adelberg, E. A., Mandel, M., and Chien Ching Chen, G., Biochem. Biophys. Res. Commun., 18, 788 (1965).
Hurwitz, J., Gold, M., and Anders, M., J. Biol. Chem., 239, 3462 (1964).
Steinberg, R. A., and Denhardt, D. T., J. Mol. Biol., 37, 525 (1968).
Gross, J. D., and Gross, M., Nature, 224, 1166 (1969).
Richardson, C. C., Schildkraut, C. L., Aposhian, H. V., and Kornberg, A., J. Biol. Chem., 239, 222 (1964).
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DE LUCIA, P., CAIRNS, J. Isolation of an E. coli Strain with a Mutation affecting DNA Polymerase. Nature 224, 1164–1166 (1969). https://doi.org/10.1038/2241164a0
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DOI: https://doi.org/10.1038/2241164a0
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