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Isolation of an E. coli Strain with a Mutation affecting DNA Polymerase

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Abstract

By testing indiscriminately several thousand colonies of mutagenized E. coli, a mutant has been isolated that on extraction proves to have less than I per cent of the normal level of DNA polymerase. The mutant multiplies normally but has acquired an increased sensitivity to ultraviolet light.

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References

  1. Lehman, I. R., Bessman, M. J., Simms, E. S., and Kornberg, A., J. Biol. Chem., 233, 163 (1958).

    CAS  PubMed  Google Scholar 

  2. Bonhoeffer, F., Z. Vererbungslehre, 98, 141 (1966).

    CAS  Google Scholar 

  3. Buttin, G., and Wright, M., Cold Spring Harbor Symp. Quant. Biol., 33, 259 (1968).

    Article  CAS  PubMed  Google Scholar 

  4. Fangman, W. L., and Novick, A., Genetics, 60, 1 (1968).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Gross, J. D., Karamata, D., and Hempstead, P. G., Cold Spring Harbor Symp. Quant. Biol., 33, 307 (1968).

    Article  CAS  PubMed  Google Scholar 

  6. Hirota, Y., Ryter, A., and Jacob, F., Cold Spring Harbor Symp. Quant. Biol., 33, 677 (1968).

    Article  CAS  PubMed  Google Scholar 

  7. Couch, J., and Hanawalt, P. C., Biochem. Biophys. Res. Commun., 29, 779 (1967).

    Article  CAS  PubMed  Google Scholar 

  8. Kanner, L., and Hanawalt, P. C., Biochim. Biophys. Acta, 157, 532 (1968).

    Article  CAS  PubMed  Google Scholar 

  9. Kornberg, A., Science, 163, 1410 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Gesteland, R. F., J. Mol. Biol., 16, 67 (1966).

    Article  CAS  PubMed  Google Scholar 

  11. Godson, G. N., and Sinsheimer, R. L., Biochim. Biophys. Acta, 149, 476 (1967).

    Article  CAS  PubMed  Google Scholar 

  12. Billen, D., Biochim. Biophys. Acta, 68, 342 (1963).

    Article  CAS  PubMed  Google Scholar 

  13. Cohen, A., Fisher, W. D., Curtiss, R., and Adler, H. I., Cold Spring Harbor Symp. Quant. Biol., 33, 635 (1968).

    Article  CAS  PubMed  Google Scholar 

  14. Hirota, Y., Jacob, F., Ryter, A., Buttin, G., and Nakai, T., J. Mol. Biol., 35, 175 (1968).

    Article  CAS  PubMed  Google Scholar 

  15. Adelberg, E. A., Mandel, M., and Chien Ching Chen, G., Biochem. Biophys. Res. Commun., 18, 788 (1965).

    Article  CAS  Google Scholar 

  16. Hurwitz, J., Gold, M., and Anders, M., J. Biol. Chem., 239, 3462 (1964).

    CAS  PubMed  Google Scholar 

  17. Steinberg, R. A., and Denhardt, D. T., J. Mol. Biol., 37, 525 (1968).

    Article  CAS  PubMed  Google Scholar 

  18. Gross, J. D., and Gross, M., Nature, 224, 1166 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  19. Richardson, C. C., Schildkraut, C. L., Aposhian, H. V., and Kornberg, A., J. Biol. Chem., 239, 222 (1964).

    CAS  PubMed  Google Scholar 

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DE LUCIA, P., CAIRNS, J. Isolation of an E. coli Strain with a Mutation affecting DNA Polymerase. Nature 224, 1164–1166 (1969). https://doi.org/10.1038/2241164a0

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  • DOI: https://doi.org/10.1038/2241164a0

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