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New Visual Markers of Antibody for Electron Microscopy

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Abstract

SINGER1 introduced ferritin-labelling of specific antibody as a means of identifying antigens in electron micrographs. This technique has been widely applied to the location of cellular, bacterial and viral antigens2. Recently we have modified the technique by preparing hybrid F(ab′)2 antibody, permitting the development of a multiple-marker system and obviating the chemical coupling of ferritin to antibody (which engenders heterogeneous products and causes considerable loss of antibody activity). In the indirect version of this modified test3, as used for the location of mouse cell surface antigens, alloantibody is applied to the viable cells in suspension, followed by hybrid antibody with one combining site for mouse γG and the other for ferritin, and finally by ferritin. Thus the reaction sequence is surface alloantigen: alloantibody (mouse γ)G : anti-mouse γG/anti-ferritin hybrid antibody : ferritin. Labelling in this way is highly specific and reproducible.

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Author notes

  1. U. HÄMMERLING

    Present address: Institut für Virologie der Medizinischen Fakultät der Justus Liebig-Universität Giessen, 6300 Giessen, Frankfurter Strasse, 87, Germany

Authors and Affiliations

  1. Divisions of Immunology and Cytology, Sloan-Kettering Institute for Cancer Research, New York, New York, 10021

    U. HÄMMERLING, T. AOKI, H. A. WOOD, L. J. OLD, E. A. BOYSE & E. DE HARVEN

  2. Boyce Thompson Institute for Plant Research, Yonkers, New York, 10701

    U. HÄMMERLING, T. AOKI, H. A. WOOD, L. J. OLD, E. A. BOYSE & E. DE HARVEN

Authors
  1. U. HÄMMERLING
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  2. T. AOKI
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  3. H. A. WOOD
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  4. L. J. OLD
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  5. E. A. BOYSE
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  6. E. DE HARVEN
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HÄMMERLING, U., AOKI, T., WOOD, H. et al. New Visual Markers of Antibody for Electron Microscopy. Nature 223, 1158–1159 (1969). https://doi.org/10.1038/2231158a0

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  • DOI: https://doi.org/10.1038/2231158a0

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