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Isolation of Polysomes from Mouse Plasmacytomas

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Abstract

THE difficulty of isolating polysomes from many tissues, including plasma cell tumours1–4, has been attributed to the release of ribonuclease (RNase) following cellular disruption. Thus the quality of poly some preparations isolated from tissues containing high concentrations of RNase should be improved by procedures that decrease the time that polysomes are exposed to the crude cell lysate.

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References

  1. Shapiro, A. L., Scharff, M. D., Maizel, J. V., and Uhr, J. W., Proc. US Nat. Acad. Sci., 56, 216 (1966).

    Article  ADS  CAS  Google Scholar 

  2. Williamson, A. R., and Askonas, B. A., J. Mol. Biol., 23, 201 (1967).

    Article  CAS  PubMed  Google Scholar 

  3. Schubert, D., Proc. US Nat. Acad. Sci., 60, 683 (1968).

    Article  ADS  CAS  Google Scholar 

  4. Mach, B., Koblet, H., and Gros, D., Proc. US Nat. Acad. Sci., 59, 445 (1968).

    Article  ADS  CAS  Google Scholar 

  5. Potter, M., Dreyer, W. J., Kuff, E. L., and McIntyre, K. R., J. Mol. Biol., 8, 814 (1964).

    Article  CAS  PubMed  Google Scholar 

  6. Potter, M., Appella, E., and Geisser, S., J. Mol. Biol., 14, 361 (1965).

    Article  CAS  PubMed  Google Scholar 

  7. Potter, M., and Fahey, J. L., J. Nat. Cancer Inst., 29, 1153 (1960).

    Google Scholar 

  8. de Petris, S., and Karlsbad, G., J. Cell. Biol., 26, 759 (1965).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Reader, R. W., and Stanners, C. P., J. Mol. Biol., 28, 211 (1967).

    Article  CAS  PubMed  Google Scholar 

  10. Martin, R. G., and Ames, B. N., J. Biol. Chem., 236, 1372 (1961).

    PubMed  CAS  Google Scholar 

  11. Becker, M. J., and Rich, A., Nature, 212, 142 (1966).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Scharff, M. D., and Uhr, J. W., Science, 148, 646 (1965).

    Article  ADS  CAS  PubMed  Google Scholar 

  13. Norton, W. L., Lewis, D., and Ziff, M., Proc. US Nat. Acad. Sci., 54, 851 (1965).

    Article  ADS  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Laboratory for Biophysical- and Immuno-Chemistry, Department of Chemistry, McGill University, Montreal

    B. K. DAVIS, T. L. DELOVITCH & A. H. SEHON

Authors
  1. B. K. DAVIS
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  2. T. L. DELOVITCH
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  3. A. H. SEHON
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DAVIS, B., DELOVITCH, T. & SEHON, A. Isolation of Polysomes from Mouse Plasmacytomas. Nature 222, 172–174 (1969). https://doi.org/10.1038/222172a0

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  • DOI: https://doi.org/10.1038/222172a0

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