Abstract
THE observation of broken chromosomes in test animals and humans treated with the hallucinogen lysergic acid diethylamide (LSD) has been well documented1–8. Although there has been some speculation about the mode of action of the drug, no specific studies of the mechanism of LSD-induced chromosomal damage have, as yet, been presented. Such studies would be most useful in determining whether LSD interacts directly with the chromosome which becomes broken or whether chromosomal breakage is the result of a series of biochemical events begun by LSD at some site distant from the chromosome. This communication describes circular dichroism experiments which indicate that LSD interacts directly with purified calf thymus DNA, probably by intercalation, causing conformational changes in the DNA. It is suggested that this observed interaction may serve as a model for the action of LSD on the chromosomal material of intact cells.
Similar content being viewed by others
References
Cohen, M. M., Marinello, M. J., and Back, N., Science, 155, 1417 (1967).
Cohen, M. M., Hirschhorn, K., and Frosch, W. A., New Engl. J. Med., 277, 1043 (1967).
Jarvik, L. F., and Kato, T., Lancet, i, 250 (1968).
Cohen, M. M., Hirschhorn, K., Verbo, S., Frosch, W. A., and Groeschal, M., Pediat. Res., (in the press).
Irwin, S., and Egozcue, J., Science, 157, 313 (1967).
Egozcue, J., Irwin, S., and Maruffo, C. A., J. Amer. Med. Assoc., 204, 214 (1968.)
Zellweger, H., McDonald, J. S., and Abbo, G., Lancet, ii, 1066 (1962).
Cohen, M. M., and Mukerjee, A. B., Nature, 219, 1972 (1968).
Fuller, W., and Waring, M. J., Ber. Bunsengesellschaft Physik. Chem., 68, 805 (1964).
Mommaerts, W. F. H. M., in Advances in Enzymology (edit by Grossman, L., and Maldane, K.), 12, 302 (Academic Press, New York, 1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
WAGNER, T. In vitro Interaction of LSD with Purified Calf Thymus DNA. Nature 222, 1170–1172 (1969). https://doi.org/10.1038/2221170b0
Received:
Issue Date:
DOI: https://doi.org/10.1038/2221170b0
- Springer Nature Limited
This article is cited by
-
LSD induced chlorophyll mutations in barley
Experientia (1978)
-
RNA in planarians (Dugesia lugubris) treated with LSD
Experientia (1973)
-
Optical Activity of LSD–DNA Mixtures
Nature (1971)
-
LSD-25 Does Not Intercalate in DNA
Nature (1971)
-
Testing of LSD-25 and related compounds for possible effects on egg-laying capacity and egg-to-adult viability inDrosophila
Experientia (1971)