Abstract
TSUKADA et al.1 have recently reported that, after denaturation, newly replicated DNA from regenerating rat liver cells apparently has a lower molecular weight than the main body of the chromosomal DNA. After pulse labelling with tritiated thymidine, they isolated the DNA by conventional methods, which extensively shear the DNA, thereby reducing its chain length, and then measured the molecular weight of the denatured DNA by sedimentation on an akaline sucrose gradient.
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References
Tsukada, K., Moriyama, T., Lynch, W. E., Lieberman, I., Nature, 220, 162 (1968).
Lett, J. T., Caldwell, I., Dean, C. J., and Alexander, P., Nature, 214, 790 (1967).
Charlesby, A., Proc. Roy. Soc., A 224, 120 (1954).
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LEHMANN, A., ORMEROD, M. Artefact in the Measurement of the Molecular Weight of Pulse Labelled DNA. Nature 221, 1053–1056 (1969). https://doi.org/10.1038/2211053b0
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DOI: https://doi.org/10.1038/2211053b0
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