Coupling of Enzymes to Cellulose using Chloro-s-triazines

Abstract

MANY methods are now available for coupling enzymes and other biologically active compounds to solid supports^1–4. Most involve the preliminary preparation of a special support or of a special derivative of a general support such as cellulose; for example, the carboxymethyl or p-amino-benzyl ether derivative. We have therefore sought coupling reagents which allow a more direct approach and have developed the use of cyanuric chloride and dichloro-s-triazines. The latter can be prepared as stable compounds with solubilizing groups such as carboxy–methoxy or carboxy–methylamino, which make them very convenient reagents.