Abstract
THERE are obvious advantages in culturing tissue cells in suspension instead of in monolayers and in using the suspension as a substrate for virus multiplication. Culture conditions for cell growth and virus multiplication can be studied and large scale production can be achieved more easily. The growth of cells from cell-lines, in suspension culture, as separate units or small clumps, has already been demonstrated. In contrast, nobody has yet succeeded in growing diploid and primary cells by this method1,2, for the cells have become aneuploid or did not grow at all in suspension culture3.
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References
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VAN WEZEL, A. Growth of Cell-strains and Primary Cells on Micro-carriers in Homogeneous Culture. Nature 216, 64–65 (1967). https://doi.org/10.1038/216064a0
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DOI: https://doi.org/10.1038/216064a0
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