Skip to main content
SpringerLink
Log in

Selective Modification of Mitochondrial Malate Dehydrogenase Activity by Changes in Ionic Strength

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

MITOCHONDRIAL malate dehydrogenase (MDH) isozymes are strongly inhibited by 10−3 molar oxalo-acetate, while under comparable conditions the cytoplasmic MDH shows no substrate inhibition1–5. This catalytic test readily distinguishes the two types of MDH isozymes2. Further refinement of catalytic discrimination between MDH isozymes was recently achieved by the determination of velocity ratios in presence of oxalo-acetate and its fluoro analogues4. The present communication describes the observation that catalytic activity of mitochondrial MDH is highly susceptible to relatively small changes in ionic strength (μ) as well as to the qualitative composition of ionic environment. On the other hand, the cytoplasmic isozyme is completely insensitive to these influences.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Davies, D. D., and Kun, E., Biochem. J., 66, 307 (1957).

    Article  CAS  Google Scholar 

  2. Delbrück, A., Zebe, A. E., and Bücher, T., Biochem. Z., 331, 273 (1959).

    Google Scholar 

  3. Kun, E., Gottwald, L. K., Fanshier, D. W., and Ayling, J. E., J. Biol. Chem., 238, 1456 (1963).

    CAS  Google Scholar 

  4. Kun, E., and Volfin, P., Biochem. Biophys. Res. Comm., 22, 187 (1966).

    Article  CAS  Google Scholar 

  5. Kun, E., in The Enzymes (edit. by Boyer, P. D., Lardy, H. A., and Myrbäck, K.), 7, 149 (Academic Press, New York, 1963).

    Google Scholar 

  6. Chilson, O. P., Kitto, B. G., and Kaplan, N. O., Proc. U.S. Nat. Acad. Sci, 53, 1006 (1965).

    Article  ADS  CAS  Google Scholar 

  7. Chilson, O. P., Kitto, B. G., Puldes, J., and Kaplan, N. O., J. Biol. Chem., 241, 2431 (1966).

    CAS  Google Scholar 

  8. Gerhart, J. C., and Schachman, H. K., Biochem., 4, 1054 (1965).

    Article  CAS  Google Scholar 

  9. Lehninger, A. L., The Mitochondrion (W. A. Benjamin, New York, 1964).

    Google Scholar 

  10. Carafoli, E., Gamble, R. L., and Lehninger, A. L., Biochem. Biophys. Res. Comm., 21, 488 (1965).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Pharmacology and Cardiovascular Research Institute, School of Medicine, University of California, San Francisco, California

    ERNEST KUN, ROBERT Z. EANES & PIERRE VOLFIN

Authors
  1. ERNEST KUN
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. ROBERT Z. EANES
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. PIERRE VOLFIN
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

KUN, E., EANES, R. & VOLFIN, P. Selective Modification of Mitochondrial Malate Dehydrogenase Activity by Changes in Ionic Strength. Nature 214, 1328–1330 (1967). https://doi.org/10.1038/2141328a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/2141328a0

  • Springer Nature Limited

This article is cited by

Search

Navigation