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Solid Phase Radioimmunoassay

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Abstract

ALL forms of radioimmunoassay used at present include a procedure to separate free and bound tracer antigen when equilibrium is reached in the incubation mixture. This separation may involve precipitation by a second antibody1,2, electrophoresis3,4, chromatoelectrophoresis5, ion exchange6, solvent fractionation7, gel filtration8,9, salt precipitation10, or adsorption to charcoal11. The need to use such formal isolation procedures increases the length and complexity of the assay and introduces a number of possible sources of error.

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Authors and Affiliations

  1. Monash University Department of Medicine, Prince Henry's Hospital, Melbourne

    KEVIN CATT & H. D. NIALL

  2. Imperial Chemical Industries of Australia and New Zealand, Ltd., Central Research Laboratories, Ascot Vale, Melbourne, Victoria

    G. W. TREGEAR

Authors
  1. KEVIN CATT
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  2. H. D. NIALL
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  3. G. W. TREGEAR
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CATT, K., NIALL, H. & TREGEAR, G. Solid Phase Radioimmunoassay. Nature 213, 825–827 (1967). https://doi.org/10.1038/213825a0

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