Isoamylases of Human Parotid Saliva

Abstract

COLUMN electrophoresis with a polyacrylamide gel was used by Muus and Vneuchak¹ to separate from crystalline human saliva amylase four protein staining bands which suggested four different molecular forms of amylase. The staining evidence was corroborated with the finding of four areas of amylase activity from gel section eluates. A more direct method with greater resolving power for detecting the multiple molecular forms of amylase has been developed for use with polyacrylamide gel columns. Preliminary studies show that there are generic differences in salivary amylase and that a polymorphism exists among human parotid salivas.