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Heterogeneity of Human Growth Hormone Preparations by Immuno-gel Filtration and Gel Filtration Electrophoresis

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Abstract

VARIOUS methods have been used for the purification of human growth hormone (HGH). The method described by Roos et al.1 uses especially mild conditions with extraction of homogenized frozen pituitary glands at pH 6.2, followed by ammonium sulphate precipitation and gel filtration on ‘Sephadex’. We sought to check the homogeneity of preparations obtained by this method which had been lyophilized and dispensed for therapeutic use. With the small amounts of material available it seemed convenient to apply thin-layer gel filtration2 on ‘Sephadex G-200’ in combination with immunodiffusion analysis (immuno-gel filtration) or with electrophoresis (gel filtration electrophoresis)3,4.

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References

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Authors and Affiliations

  1. Department of Pediatrics, Karolinska Institute, Crown Princess Louisa's Children's Hospital, Stockholm, Sweden

    L. Å. HANSON

  2. Institute of Biochemistry, University of Uppsala, Sweden

    P. Roos

  3. Department of Medical Biochemistry, University of Göteborg, Sweden

    L. RYMO

Authors
  1. L. Å. HANSON
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  2. P. Roos
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  3. L. RYMO
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HANSON, L., Roos, P. & RYMO, L. Heterogeneity of Human Growth Hormone Preparations by Immuno-gel Filtration and Gel Filtration Electrophoresis. Nature 212, 948–949 (1966). https://doi.org/10.1038/212948a0

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