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Hexokinase Isozyme Patterns of Human Erythrocytes and Leucocytes

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Abstract

HEXOKINASE catalyses the initial phosphorylation of glucose in the glycolytic pathway of carbohydrate metabolism. This step appears to be rate limiting in glycolysis of the human erythrocyte1,2. Quantitative studies of the activity of hexokinase have been carried out with human erythrocytes1–5, but the qualitative characteristics of the enzyme from red cells or white cells have not been evaluated by electrophoretic methods. This communication describes a method for investigating the hexokinase isozyme patterns of haemolysates and leucocyte sonicates, and presents our observations on these patterns in certain population groups.

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Authors and Affiliations

  1. Departments of Medicine (Simpson Memorial Institute) and Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan

    GARY M. EATON, GEORGE J. BREWER & RICHARD E. TASHIAN

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  1. GARY M. EATON
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  2. GEORGE J. BREWER
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  3. RICHARD E. TASHIAN
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EATON, G., BREWER, G. & TASHIAN, R. Hexokinase Isozyme Patterns of Human Erythrocytes and Leucocytes. Nature 212, 944–946 (1966). https://doi.org/10.1038/212944b0

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