Skip to main content
SpringerLink
Log in

Reconstruction in vivo of Irradiated Escherichia coli Deoxyribonucleic Acid; the Rejoining of Broken Pieces

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

DNA is presumably the primary site at which radiation damage has biological consequences1. Although lesions in single strands can be repaired after ultra-violet exposure2,3, it has not been shown conclusively that X-ray damage to bacterial DNA can be repaired. In order to study the latter problem, we have devised a method for assaying long pieces of single stranded DNA in order to facilitate the detection of a small number of breaks in single strands induced by X-rays.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Haynes, R. H., in Physical Processes in Radiation Biology, 51, edit. by Augenstein L., Mason, R., and Rosenberg, B. (Academic Press, New York, 1964).

    Book  Google Scholar 

  2. Setlow, R. B., and Carrier, W. L., Proc. U.S. Nat. Acad. Sci., 51, 226 (1964).

    Article  ADS  CAS  Google Scholar 

  3. Boyce, R. P., and Howard-Flanders, P., Proc. U.S. Nat. Acad. Sci., 51, 293 (1964).

    Article  ADS  CAS  Google Scholar 

  4. Adler, H. I., Adv. Radiobiol., 2 (Academic Press, Inc., New York, in the press).

  5. Hill, R. F., and Simpson, E., J. Gen. Microbiol., 24, 1 (1961).

    Article  CAS  Google Scholar 

  6. Cairns, J., J. Mol. Biol., 6, 208 (1963).

    Article  CAS  Google Scholar 

  7. Boyce, R. P., and Setlow, R. B., Biochim. Biophys. Acta, 61, 618 (1962).

    CAS  Google Scholar 

  8. Fraser, D. H., Mahler, R., Shug, A. L., and Thomas, C. A., Proc. U.S. Nat. Acad. Sci., 43, 939 (1957).

    Article  ADS  CAS  Google Scholar 

  9. Bollum, F. J., J. Biol. Chem., 234, 2733 (1959).

    CAS  PubMed  Google Scholar 

  10. Burgi, E., and Hershey, A. D., Biophys. J., 3, 309 (1963).

    Article  ADS  CAS  Google Scholar 

  11. Studier, F. W., J. Mol. Biol., 11, 373 (1965).

    Article  CAS  Google Scholar 

  12. Freifelder, D., Proc. U.S. Nat. Acad. Sci., 54, 128 (1965).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

    R. A. MCGRATH & R. W. WILLIAMS

Authors
  1. R. A. MCGRATH
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. W. WILLIAMS
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

MCGRATH, R., WILLIAMS, R. Reconstruction in vivo of Irradiated Escherichia coli Deoxyribonucleic Acid; the Rejoining of Broken Pieces. Nature 212, 534–535 (1966). https://doi.org/10.1038/212534a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/212534a0

  • Springer Nature Limited

This article is cited by

Search

Navigation