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Effects of Actinomycin D and Puromycin on Chromosomes of Cultured Human Leucocytes

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Abstract

ACTINOMYCIN D is a potent antibiotic with cytotoxic properties in tissue cultures1–3 which has been used in the treatment of human neoplasms4–9. The action of actinomycin may depend on its capacity to bind with DNA, thus inhibiting RNA synthesis10. It has also been suggested that it is the synthesis of the DNA-primed RNA which is inhibited11. Puromycin, also an antibiotic, is believed to inhibit protein synthesis in a wide variety of organisms and tissues by causing premature release of incomplete polypeptide chains from the ribosomal system12–20.

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Author notes

  1. JOSÉ CARNEIRO LEÃO: Postdoctoral research fellow, U.S. Public Health Service, National Institutes of Health.

Authors and Affiliations

  1. Genetic and Endocrine Unit, Department of Pediatrics, State University of New York, Upstate Medical Center, Syracuse, New York

    RICHARD L. NEU, JOSÉ CARNEIRO LEÃO & LYTT I. GARDNER

Authors
  1. RICHARD L. NEU
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  2. JOSÉ CARNEIRO LEÃO
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  3. LYTT I. GARDNER
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NEU, R., LEÃO, J. & GARDNER, L. Effects of Actinomycin D and Puromycin on Chromosomes of Cultured Human Leucocytes. Nature 211, 881–882 (1966). https://doi.org/10.1038/211881a0

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