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Comparison of Methods for separating Blood Plasma Lipoproteins

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Abstract

THE analysis of many samples of blood by both the ultracentrifugal1 and by Cohn's micro-method 10 (ref. 2) has led us to observe that the cholesterol in the β-lipo-protein fraction (I + II + III) exceeds that in the low-density fraction (d < 1.063 g/ml.) of the same plasma, by an average of 17 mg/100 ml. plasma (0–35 mg per cent). Moreover, the ‘high density’ cholesterol-level (HDL) was greater than that of the α-lipoproteins by a corresponding amount (Table 1).

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References

  1. DeLalla, O., and Gofman, J. W., Methods of Biochemical Analysis, 1, edit. by Glick, D. (Interscience, New York, 1954).

    Google Scholar 

  2. Cohn, E. J., Gurd, F. R. N., Surgenor, D. M., Barnes, B. A., Brown, R. K., Derouaux, G., Gillespie, J. M., Kahnt, F. W., Lever, W. F., Liu, C. H., Mittleman, D., Mouton, R. F., Schmid, K., and Uroma, E., J. Amer. Chem. Soc., 72, 465 (1950).

    Article  CAS  Google Scholar 

  3. Barclay, M., Cogin, G. E., Escher, G. C., Kaufman, R. J., Kidder, E. D., and Peterman, M. L., Cancer, 8, 253 (1955).

    Article  CAS  Google Scholar 

  4. Carlson, L. A., Clin. Chim. Acta, 5, 528 (1960).

    Article  CAS  Google Scholar 

  5. Mills, G. L., and Wilkinson, P. A., Clin. Chim. Acta, 8, 701 (1963).

    Article  CAS  Google Scholar 

  6. Ray, B. R., Davisson, E. C., and Crespi, H. L., J. Amer. Chem. Soc., 58, 841 (1954).

    CAS  Google Scholar 

  7. Peterman, M. L., Barclay, M., Escher, G., and Kaufman, R., The Lipoproteins, Methods and Clinical Significance, edit. by Homburger, F., and Bernfeld, P.(S. Karger, New York, 1958).

    Google Scholar 

  8. Hayashi, S., Lindgren, F., and Nichols, A., J. Amer. Chem. Soc., 81, 3793 (1959).

    Article  CAS  Google Scholar 

  9. Brown, R., deLalla, L., and Kauffman, D., Clin. Chim., 1, 83 (1955).

    Article  CAS  Google Scholar 

  10. Lindgren, F., and Nichols, A. V., The Plasma Proteins, 2, edit. by Putnam, F. W. (Academic Press, New York, 1960).

    Google Scholar 

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MILLS, G., TAYLAUR, C. & WILKINSON, P. Comparison of Methods for separating Blood Plasma Lipoproteins. Nature 206, 191–192 (1965). https://doi.org/10.1038/206191a0

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