Abstract
THAT the iron atom of cytochrome c is bound by the imidazole rings of two histidine residues was first suggested by Theorell and Akesson1. An investigation of the effect of pH on the equilibria between ferricytochrome c and ionic ligands such as CN− and N−3 provided physicochemical evidence for the presence of at least one Fe-histidine bond in the oxidized state of the molecule2. If this bond is broken, as for example when a complex of ferricytochrome c with a ligand is formed, the imidazole nitrogen liberated from iron co-ordination should become available for reactions that do not take place with the native molecule. Further confirmation of this structure should thus be possible by more direct chemical observations.
Similar content being viewed by others
References
Theorell, H., and Akesson, A., J. Amer. Chem. Soc., 63, 1812, 1818, 1820 (1941).
George, P., Glauser, S. C., and Schejter, A., in Molecular Basis for Enzyme Action and Inhibition, edit. by Desnuelle, P., Proc. Fifth Cong. Biochem., Moscow, 1961, 4, 192 (Pergamon Press, Oxford, 1963).
George, P., and Lyster, R. L. J., Proc. U.S. Nat. Acad. Sci., 44, 1013 (1958).
See Lemberg, R., and Legge, J. W., Hematin Compounds and Bile Pigments (Interscience, New York, 1949).
Scheller, W., Salewski, A., and Jung, F., Biochem. Z., 326, 288 (1955).
George, P., Lyster, R. L. J., and Beetlestone, J., Nature, 181, 1534 (1958).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SCHEJTER, A., GEORGE, P. Production of a ‘Cytochrome c’ with Myoglobin-like Properties by Alkylating the Cyanide Complex with Bromoacetate. Nature 206, 1150–1151 (1965). https://doi.org/10.1038/2061150a0
Issue Date:
DOI: https://doi.org/10.1038/2061150a0
- Springer Nature Limited