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Use of 2,4-Dinitrophenylhydrazine for the Estimation of Micro Amounts of Carbonyls

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Abstract

THE use of 2,4-dinitrophenyl hydrazine (2,4-DNP hydrazine) in acid solution as a reagent for the estimation of methyl ketones and other carbonyls is common despite the fact that the reaction is apparently non-quantitative1–3. Between 20 and 90 per cent recoveries of methyl ketones from aqueous solution have been reported4,5. Wong, Patton and Forss4 attributed the low recovery of 2-pentanone (25 per cent) from whole milk by steam distillation under reduced pressure to the non-completion of the reaction with the reagent. Haverkamp Begemann and de Jong5 concluded that the method of shaking had a marked influence on the yield of 2,4-DNP hydrazone, and by use of ‘Celite’ /2,4-DNP hydrazine column to ensure more intimate contact between the reagent and the carbonyl compound they achieved practically complete conversion into the 2,4-DNP hydrazone.

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Authors and Affiliations

  1. Dairy Research Institute, Palmerston North, New Zealand

    R. C. LAWRENCE

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  1. R. C. LAWRENCE
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LAWRENCE, R. Use of 2,4-Dinitrophenylhydrazine for the Estimation of Micro Amounts of Carbonyls. Nature 205, 1313–1314 (1965). https://doi.org/10.1038/2051313a0

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