Skip to main content

Advertisement

Log in

Stimulation of Steroid C-11β Hydroxylase Activity in Adrenal Homogenates by Cyclic 3′,5′-Adenosine Monophosphate

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

STEROID biosynthesis in the mammalian adrenal cortex is dependent on the activity of a number of complex enzyme systems, including several hydroxylases which require NADPH as cofactor1. NADPH is continuously generated from NADP within the adrenal cortex by the action of several dehydrogenases acting on appropriate substrates2. Of the latter, glucose-6-phosphate appears to be the most important3. Haynes, Koritz and Péron4 demonstrated that adenosine-3′,5′-monophosphate (cyclic 3′,5′-AMP) enhanced the production of corticosterone from endogenous precursors by surviving rat adrenal sections. Since cyclic 3′,5′-AMP also stimulated phosphorylase activity in adrenal slices, the effect of the nucleotide on corticosteroidogenesis was apparently related to increased provision of glucose-6-phosphate5. Of added significance was the observation by Haynes5,6 that ACTH resulted in the accumulation in adrenal slices of cyclic 3′,5′-AMP, which presumably was responsible for the resulting stimulation of corticosteroidogenesis. Attempts to demonstrate a stimulatory effect of cyclic 3′,5′-AMP on corticosteroidogenesis from endogenous substrates in adrenal homogenates have hitherto been unsuccessful4,7. In the investigations recorded here, addition of this nucleotide to rat adrenal homogenates markedly stimulated C-11β hydroxylation of added progesterone and 11-deoxycorticosterone, but appeared to have no effect on C-21 hydroxylation of progesterone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Samuels, L. T., in Metabolic Pathways, edit. by Greenberg, D. M., 1, 431 (Academic Press, Inc., New York, 1960).

    Google Scholar 

  2. Koritz, S. B., and Péron, F. G., J. Biol. Chem., 230, 343 (1958).

    CAS  PubMed  Google Scholar 

  3. McKerns, K. W., Biochim. Biophys. Acta, 71, 710 (1963).

    Article  CAS  Google Scholar 

  4. Haynes, jun., R. C., Koritz, S. B., and Péron, F. G., J. Biol. Chem., 234, 1421 (1959).

    CAS  PubMed  Google Scholar 

  5. Haynes, jun., R. C., J. Biol. Chem., 233, 1220 (1958).

    CAS  PubMed  Google Scholar 

  6. Haynes, jun., R. C., and Berthet, L., J. Biol. Chem., 225, 115 (1957).

    CAS  PubMed  Google Scholar 

  7. Koritz, S. B., and Péron, F. G., J. Biol. Chem., 234, 3122 (1959).

    CAS  PubMed  Google Scholar 

  8. Roberts, S., Seto, K., and Hanking, B. H., J. Neurochem., 9, 493 (1962).

    Article  CAS  Google Scholar 

  9. Lisboa, B. P., Acta Endocrinol., 43, 47 (1963).

    Article  CAS  Google Scholar 

  10. Sease, J. W., J. Amer. Chem. Soc., 70, 3630 (1948).

    Article  CAS  Google Scholar 

  11. Makoff, R., Roberts, S., and Fowler, D. D., J. Biol. Chem. (in the press).

  12. Makoff, R., and Roberts, S., Biochim. Biophys. Acta (in the press).

  13. Grant, J. K., in ACTH, Proc. Sheffield Corticotropin Conf., edit. by West, H. F., 56 (Ferring, A. B., Malmo, 1960).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

ROBERTS, S., CREANGE, J. & FOWLER, D. Stimulation of Steroid C-11β Hydroxylase Activity in Adrenal Homogenates by Cyclic 3′,5′-Adenosine Monophosphate. Nature 203, 759–761 (1964). https://doi.org/10.1038/203759a0

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/203759a0

  • Springer Nature Limited

This article is cited by

Navigation