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Quantitative Spectrophotometric Determination of Indolyl-3-acetic Acid

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Abstract

INDOLYL-3-ACETIC acid (IAA) can be determined either by non-specific biological methods1 or by chemical methods2. Most of the quantitative work with auxins has been based on bioassays. This is probably due to their high sensitivity, and because larger amounts (300 g) of plant tissue are necessary to maintain precision when using chemical methods8. This communication describes a Spectrophotometric technique which offers a means of precise, sensitive, quantitative estimation of IAA from small amounts of plant material.

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References

  1. Larsen, P., Encyclopedia of Plant Physiology, edit. by Ruhland, W., 14, 521 (Springer-Verlag, 1961).

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  2. Bentley, J. A., Encyclopedia of Plant Physiology, edit. by Ruhland, W., 14, 501 (Springer-Verlag, 1961).

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  3. Vlitos, A. J., and Meudt, W., Contrib. Boyce Thompson Inst., 17, 401 (1954).

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  4. Harley-Mason, J., and Archer, A. A. P. G., Biochem. J., 69, 60 (1958).

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Authors and Affiliations

  1. Department of Plant Science, University of Alberta, Edmonton, Canada

    R. A. FLETCHER & SAUL ZALIK

Authors
  1. R. A. FLETCHER
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  2. SAUL ZALIK
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FLETCHER, R., ZALIK, S. Quantitative Spectrophotometric Determination of Indolyl-3-acetic Acid. Nature 199, 903–904 (1963). https://doi.org/10.1038/199903b0

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  • DOI: https://doi.org/10.1038/199903b0

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