Skip to main content
SpringerLink
Log in

Adsorption of Rabbit Antibody by Rat Tissue Mast Cells and Other Cells in vitro

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

FROM observations obtained on guinea pig mesenteries dissected out from animals which had been passively sensitized in vivo with rabbit anti-ovalbumin, Humphrey and Mota1 concluded that the antibody is reversibly adsorbed to the mast cell surface. When normal tissue mast cells isolated from the peritoneal fluid of albino rats were incubated with rabbit anti-human γ-globulin and afterwards treated with human γ-globulin characteristic morphological changes and release of some of the stored histamine occurred at the same time2. None of these authors has been able to provide direct evidence for the presence of cell-fixed antibody on mast cells by means of the fluorescent antibody technique. However, White3, using this technique, demonstrated recently that some of the antibody-containing fractions from rabbit sera were indeed fixed to the mast cell surface in guinea pigs. The work reported here deals with quantitative aspects of antibody adsorption by rat mast cells as compared with the adsorptive capacity of other tissue cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Humphrey, J. H., and Mota, I., Immunol., 2, 31 (1959).

    CAS  Google Scholar 

  2. Keller, R., and Schwarz, M., Schweiz. Med. Wochenschr., 91, 1196 (1961).

    CAS  PubMed  Google Scholar 

  3. White, R. G., Transact. Colleg. int. Allerg., Freiburg, 1962. Intern. Arch. Allergy (in the press).

  4. Keller, R., Pathol. Microbiol., 24, 932 (1961).

    CAS  Google Scholar 

  5. Boyden, S. V., and Sorkin, E., Immunol., 3, 272 (1960); 4, 244 (1961).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dermatologische Universitätsklinik, Zurich

    R. KELLER

  2. Schweizerisches Forschungsinstitut, Medizinische Abteilung, Davos

    E. SORKIN

Authors
  1. R. KELLER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. SORKIN
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

KELLER, R., SORKIN, E. Adsorption of Rabbit Antibody by Rat Tissue Mast Cells and Other Cells in vitro. Nature 199, 709–710 (1963). https://doi.org/10.1038/199709a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/199709a0

  • Springer Nature Limited

This article is cited by

Search

Navigation