Abstract
THE peroxidatic activity of the red blood pigment can be inhibited by cyanide in the case of methæmoglobin (HbIII), but not in the case of oxyhæmoglobin (HbII) (ref. 1). It seemed promising to use this difference for a differential staining procedure of HbII- and HbIII-containing red cells by first adding cyanide to the heparinized or citrated blood in question and by staining smears prepared from it by a peroxidase reaction.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Betke, K., Biochem. Z., 321, 271 (1951).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KLEIHAUER, E., BETKE, K. Elution Procedure for the Demonstration of Methæmoglobin in Red Cells of Human Blood Smears. Nature 199, 1196–1197 (1963). https://doi.org/10.1038/1991196b0
Issue Date:
DOI: https://doi.org/10.1038/1991196b0
- Springer Nature Limited
This article is cited by
-
A novel cytofluorometric assay for the detection and quantification of glucose-6-phosphate dehydrogenase deficiency
Scientific Reports (2012)
-
Diagnosis of red blood cell enzymopathies in infants, children and adolescents
The Indian Journal of Pediatrics (1987)
-
Die Glutathionreduktion in Erythrocyten von Gesunden und Enzymdefekttr�gern
Klinische Wochenschrift (1970)
-
Über zwei neue Fälle von hereditärer nichtsphärocytärer hämolytischer Anämie bei Glucose-6-Phosphat-Dehydrogenase-Defekt in einer norddeutschen Familie
Klinische Wochenschrift (1968)
-
Kongenitale nichtsphärozytäre hämolytische Anämien durch Mangel an Glucose-6-Phosphat-Dehydrogenase der Erythrozyten in einer japanischen Familie
Blut Zeitschrift für die Gesamte Blutforschung (1968)