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Cellular Uptake of Chloramphenicol as revealed by Fluorescence Microscopy

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Abstract

As a preliminary to investigations of the effects of chloramphenicol on mammalian cells in culture, some means of measuring intracellular uptake of the drug was sought. The fluorescence of tetracycline has been used to observe its localization within cells1,2; an investigation was undertaken to see if similar methods could be applied to the examination of chloramphenicol.

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References

  1. Rall, D. P., Loo, T. L., Lane, M., and Kelly, M. G., J. Nat. Cancer Inst., 19, 79 (1957).

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  2. DuBuy, H. G., and Showacre, J. L., Science, 133, 196 (1961).

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  3. Felsenheld, H., and Carter, C. E., J. Pharmacol. and Exp. Therapeut., 132, 1 (1961).

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Author notes

  1. LEILA DIAMOND

    Present address: Institute of Virology, University of Glasgow,

Authors and Affiliations

  1. Sloan-Kettering Institute for Cancer Research,

    LEILA DIAMOND

  2. the Sloan-Kettering Division of Cornell University Medical College, New York

    LEILA DIAMOND

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  1. LEILA DIAMOND
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DIAMOND, L. Cellular Uptake of Chloramphenicol as revealed by Fluorescence Microscopy. Nature 199, 90–91 (1963). https://doi.org/10.1038/199090a0

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