Abstract
THE separation of bovine carboxyhæmoglobin and whale carboxymyoglobin by ion exchange chromatography on DEAE-cellulose has been described by Brown1. Recently, Flodin2 has discussed the fractionation of some peptides and proteins utilizing the molecular sieve action of cross-linked dextran gels (‘Sephadex’). In the course of our work on the chromatography and microheterogeneity of myoglobin, we have found that oxyhæmoglobin and ferrimyoglobin can be easily separated on ‘Sephadex G-75’ and ‘Sephadex G-50’ columns.
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References
Brown, W. D., J. Biol. Chem., 236, 2238 (1961).
Flodin, P., Dextran Gels and their Applications in Gel Filtration (Pharmacia, Uppsala, Sweden, 1962).
Drabkin, D. L., J. Biol. Chem., 164, 703 (1946).
Huisman, T. H. J., and Prins, H. K., J. Lab. Clin. Med., 46, 255 (1955).
Perutz, M. F., Brookhaven Symp. Biol., No. 13, Protein Structure and Function, 165 (Brookhaven National Laboratory, Upton, New York, 1960).
Edmundson, A. B., and Hirs, C. H. W., Nature, 190, 663 (1961).
Hughes, W. L., in The Proteins, edit. by Neurath, H., and Bailey, K., 2, B, 720 (Academic Press, New York, 1954).
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AWAD, E., CAMERON, B. & KOTITE, L. Chromatographic Separation of Hæmoglobin and Myoglobin on ‘Sephadex’ Gel. Nature 198, 1201–1202 (1963). https://doi.org/10.1038/1981201a0
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DOI: https://doi.org/10.1038/1981201a0
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