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Increase of Yield of Serum from Samples of Blood

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Abstract

THE usual procedure for obtaining serum from clotted blood is to detach (rim) the clot from the collecting vessel, allow the clot to retract for several hours, and centrifuge at 500–1,000g. The procedure is not only time-consuming, but often results in much hæmolysis ; in addition the yields of serum vary greatly from sample to sample. By using stronger centrifugal force and thromboplastin, I have been able to obtain serum in high yields, within 1 h after bleeding, and with a minimum of hæmolysis.

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References

  1. Hawk, P. B., Oser, B. L., and Summerson, W. H., Practical Physiological Chemistry, thirteenth ed., 490 (McGraw-Hill Book Co., New York, 1954).

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  2. Hirata, A. A., Amer. J. Vet. Res., 22, 158 (1961).

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  3. Budtz-Olsen, O. E., Clot Retraction (C. C. Thomas, Springfield, Illinois, 1951).

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Authors and Affiliations

  1. National Institutes of Health, National Cancer Institute, Bethesda, 14, Maryland

    ARTHUR A. HIRATA

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  1. ARTHUR A. HIRATA
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HIRATA, A. Increase of Yield of Serum from Samples of Blood. Nature 198, 96–97 (1963). https://doi.org/10.1038/198096b0

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