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Preparation of Antisera to Human Folliclestimulating Hormone

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Abstract

WE have previously reported the preparation of antisera to fractions of human pituitary follicle-stimulating hormone which inhibited the activity of homologous antigens in mice and agglutinated red cells coated with the antigens, but they were ineffective against human chorionic gonadotrophin in rats1. The antisera have since been found to cross-react with human chorionic gonadotrophin in the red cell technique and when absorbed with human chorionic gonadotrophin they no longer agglutinated red cells coated with follicle-stimulating hormone. The antibodies, therefore, are not specific for follicle-stimulating hormone and we have now prepared antisera to new fractions.

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References

  1. Butt, W. R., Crooke, A. C., and Cunningham, F. J., Biochem. J., 81, 596 (1961); Ciba Found. Coll. Endocrinol., 14, 310 (1962).

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  2. Butt, W. R., Crooke, A. C., and Cunningham, F. J., J. Endocrinol. (in the press).

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Authors and Affiliations

  1. United Birmingham Hospitals, Department of Clinical Endocrinology, Birmingham and Midland Hospital for Women, Showell Green Lane, Birmingham, 11

    W. R. BUTT, A. C. CROOKE, F. J. CUNNINGHAM & ANNELIESE WOLF

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  1. W. R. BUTT
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  2. A. C. CROOKE
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  3. F. J. CUNNINGHAM
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  4. ANNELIESE WOLF
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BUTT, W., CROOKE, A., CUNNINGHAM, F. et al. Preparation of Antisera to Human Folliclestimulating Hormone. Nature 197, 388–389 (1963). https://doi.org/10.1038/197388b0

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