Abstract
HELL et al.1 have directed the attention of research workers to the fact that errors may result from the use of high specific activity radioactive compounds when the intracellular concentration of the corresponding metabolites is very low. In particular they mentioned tritiated thymidine and showed that the apparent effects of irradiation on the incorporation of this compound by Ehrlich ascites cells depended on whether tritiated thymidine of high or low specific activity was used. They ascribed this effect to differences in pool size in the irradiated cells.
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References
Hell, E., Berry, R. J., and Lajtha, L. G., Nature, 185, 47 (1960).
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Mendelsohn, M., Ph.D., thesis, University of Cambridge.
Newton, A. A., and Stoker, M. G. P., Virology, 5, 549 (1958).
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NEWTON, A., DENDY, P., SMITH, C. et al. A Pool Size Problem associated with the Use of Tritiated Thymidine. Nature 194, 886–887 (1962). https://doi.org/10.1038/194886a0
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DOI: https://doi.org/10.1038/194886a0
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